VISTA/B7-H5/PD-1H Antibody (MH5A) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11973

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse

Applications

Block/Neutralize, ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Lambda Clone # MH5A

Format

Azide and BSA Free

Concentration

1 mg/ml

View all VISTA/B7-H5/PD-1H Antibodies »

Product Specifications

Immunogen

The antibody was generated by immunising Armenian hamsters with mVISTA/B7-H5/PD-1HIg.

Specificity

This antibody specifically binds VISTA/B7-H5/PD-1H.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Lambda

Scientific Data Images for VISTA/B7-H5/PD-1H Antibody (MH5A) - Chimeric - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: VISTA/B7-H5/PD-1H Antibody (MH5A) - Chimeric - Azide and BSA Free [NBP3-11973]

Immunocytochemistry/ Immunofluorescence: VISTA/B7-H5/PD-1H Antibody (MH5A) - Chimeric - Azide and BSA Free [NBP3-11973]

Immunocytochemistry/Immunofluorescence: VISTA/B7-H5/PD-1H Antibody (MH5A) - Chimeric [NBP3-11973] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of MH5A (NBP3-11973) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11973, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody followed by Alexa Fluor(R) 488 secondary antibody.
ELISA: VISTA/B7-H5/PD-1H Antibody (MH5A) - Chimeric - Azide and BSA Free [NBP3-11973]

ELISA: VISTA/B7-H5/PD-1H Antibody (MH5A) - Chimeric - Azide and BSA Free [NBP3-11973]

ELISA: VISTA/B7-H5/PD-1H Antibody (MH5A) - Chimeric [NBP3-11973] - Binding curve of the rabbit chimeric version of the anti-VISTA antibody MH5A (NBP3-11973; blue line) to an ELISA plate coated with mouse VISTA-Fc fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 10000 to 1.7 ng/ml was performed using NBP3-11973. For signal detection, a 1:4000 dilution of anti-rabbit IgG1 HRP antibody was used.

Applications for VISTA/B7-H5/PD-1H Antibody (MH5A) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: VISTA/B7-H5/PD-1H

VISTA, also known as B7-H5, PD-1H, platelet receptor Gi24, Dies1, and SISP1, is a transmembrane glycoprotein that promotes both MT1-MMP expression and the MT1-MMP mediated activation of MMP-2. VISTA supports the differentiation of embryonic stem cells (ESC) and enhances BMP-4 induced signaling in ESC but is also downregulated following BMP-4 exposure. It binds to BMP-4 directly and also associates with the type I BMP receptor Activin RIB/ALK-4. VISTA is expressed on the surface of naïve CD4+ T cells, regulatory T cells, and adipocytes. It is upregulated in vivo on activated monocytes and dendritic cells. VISTA inhibits CD4+ and CD8+ T cell proliferation and their production of IL-2 and IFN-gamma. Its expression on tumor cells attenuates the anti-tumor immune response and enables more rapid tumor progression. In contrast, VISTA limits disease progression in the autoimmune disease model EAE.

Alternate Names

4632428N05Rik, B7-H5, C10orf54, Dies1, Gi24, PD-1H, PD1H, SISP1, VSIR

Gene Symbol

VSIR

Additional VISTA/B7-H5/PD-1H Products

Product Documents for VISTA/B7-H5/PD-1H Antibody (MH5A) - Chimeric - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VISTA/B7-H5/PD-1H Antibody (MH5A) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.