VISTA/B7-H5/PD-1H Antibody (onvatilimab) - Humanized

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-27970

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Functional

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # onvatilimab

Concentration

LYOPH mg/ml

View all VISTA/B7-H5/PD-1H Antibodies »

Product Specifications

Immunogen

B7-H5 / VISTA

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Endotoxin Level

< 0.001EU/ug,determined by LAL method.

Description

Expressed from CHO. The heavy chain type is huIgG1, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.

Also known as 'onvatilimab'.

Upon receipt, store immediately at -20C or lower for 24 months in a lyophilized state. - 80C for 3 months after reconstitution. Avoid repeated freeze-thaw cycles.

Scientific Data Images for VISTA/B7-H5/PD-1H Antibody (onvatilimab) - Humanized

VISTA/B7-H5/PD-1H Antibody (onvatilimab) - Humanized

ELISA: VISTA/B7-H5/PD-1H Antibody (onvatilimab) - Humanized[NBP3-27970] -

Immobilized human B7-H5 / VISTA His at 2 ug/mL can bind VISTA/B7-H5/PD-1H Antibody (onvatilimab) - Humanized, EC50=0.01513 ug/mL.
VISTA/B7-H5/PD-1H Antibody (onvatilimab) - Humanized

Flow Cytometry: VISTA/B7-H5/PD-1H Antibody (onvatilimab) - Humanized[NBP3-27970] -

Human B7H5 CHO-K cells were stained with VISTA/B7-H5/PD-1H Antibody (onvatilimab) - Humanized and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50=0.9471 ug/mL.

Applications for VISTA/B7-H5/PD-1H Antibody (onvatilimab) - Humanized

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

Formulation

Lyophilized from 25mM histidine, 8% sucrose, 0.01% Tween80 (pH6.2)

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: VISTA/B7-H5/PD-1H

VISTA, also known as B7-H5, PD-1H, platelet receptor Gi24, Dies1, and SISP1, is a transmembrane glycoprotein that promotes both MT1-MMP expression and the MT1-MMP mediated activation of MMP-2. VISTA supports the differentiation of embryonic stem cells (ESC) and enhances BMP-4 induced signaling in ESC but is also downregulated following BMP-4 exposure. It binds to BMP-4 directly and also associates with the type I BMP receptor Activin RIB/ALK-4. VISTA is expressed on the surface of naïve CD4+ T cells, regulatory T cells, and adipocytes. It is upregulated in vivo on activated monocytes and dendritic cells. VISTA inhibits CD4+ and CD8+ T cell proliferation and their production of IL-2 and IFN-gamma. Its expression on tumor cells attenuates the anti-tumor immune response and enables more rapid tumor progression. In contrast, VISTA limits disease progression in the autoimmune disease model EAE.

Alternate Names

4632428N05Rik, B7-H5, C10orf54, Dies1, Gi24, PD-1H, PD1H, SISP1, VSIR

Gene Symbol

VSIR

UniProt

Q9H7M9 (Human)

Additional VISTA/B7-H5/PD-1H Products

Product Documents for VISTA/B7-H5/PD-1H Antibody (onvatilimab) - Humanized

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for VISTA/B7-H5/PD-1H Antibody (onvatilimab) - Humanized

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.