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Vitamin D BP Antibody (VDBP/4482) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14006

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # VDBP/4482

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Summary for Vitamin D BP Antibody (VDBP/4482) - Azide and BSA Free

Immunogen

Recombinant fragment (around aa 35-175) of human Vitamin D BP protein (exact sequence is proprietary) (Uniprot: P02774)

Localization

Secreted

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13924).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Vitamin D BP Antibody (VDBP/4482) - Azide and BSA Free

Protein Array: Vitamin D BP Antibody (VDBP/4482)Azide and BSA Free [NBP3-14006]

Protein Array: Vitamin D BP Antibody (VDBP/4482)Azide and BSA Free [NBP3-14006]

Protein Array: Vitamin D BP Antibody (VDBP/4482) - Azide and BSA Free [NBP3-14006] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Vitamin D BP Antibody (VDBP/4482).
Vitamin D BP Antibody (VDBP/4482) - Azide and BSA Free

Vitamin D BP Antibody (VDBP/4482)

IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Staining using Vitamin D BP antibody (VDBP/4482) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Applications for Vitamin D BP Antibody (VDBP/4482) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Vitamin D BP

Vitamin D Binding Protein is a secreted multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and urine, and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane bound immunoglobulin on the surface of B lymphocytes and with IgG fc receptor on the membranes of T lymphocytes.

Long Name

Vitamin D Binding Protein

Alternate Names

DBP, DBP-MAF, Gc, Gc-MAF, VDBG, VDBP

Gene Symbol

GC

Additional Vitamin D BP Products

Product Documents for Vitamin D BP Antibody (VDBP/4482) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Vitamin D BP Antibody (VDBP/4482) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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