Vitamin D BP Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20863
![Western Blot: Vitamin D BP Antibody [NBP2-20863]](https://resources.bio-techne.com/images/products/Vitamin-D-Binding-Protein-Antibody-Western-Blot-NBP2-20863-img0001.jpg "Western Blot: Vitamin D BP Antibody [NBP2-20863]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Vitamin D BP Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Vitamin D BP. The exact sequence is proprietary.
Localization
Secreted
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Vitamin D BP Antibody
![Western Blot: Vitamin D BP Antibody [NBP2-20863]](https://resources.bio-techne.com/images/products/Vitamin-D-Binding-Protein-Antibody-Western-Blot-NBP2-20863-img0002.jpg "Western Blot: Vitamin D BP Antibody [NBP2-20863]")
Western Blot: Vitamin D BP Antibody [NBP2-20863]
Western Blot: Vitamin D Binding Protein Antibody [NBP2-20863] - Sample (30 ug of whole cell lysate) A: Jurkat B: K562 10% SDS PAGE gel, diluted at 1:1000.![Immunocytochemistry/ Immunofluorescence: Vitamin D BP Antibody [NBP2-20863]](https://resources.bio-techne.com/images/products/Vitamin-D-BP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20863-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Vitamin D BP Antibody [NBP2-20863]")
Immunocytochemistry/ Immunofluorescence: Vitamin D BP Antibody [NBP2-20863]
Immunocytochemistry/Immunofluorescence: Vitamin D BP Antibody [NBP2-20863] - Vitamin D BP antibody detects Vitamin D BP protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Vitamin D BP protein stained by Vitamin D BP antibody diluted at 1:500. Blue: Hoechst 33342 staining.![Immunohistochemistry-Paraffin: Vitamin D BP Antibody [NBP2-20863]](https://resources.bio-techne.com/images/products/Vitamin-D-Binding-Protein-Antibody-Immunohistochemistry-Paraffin-NBP2-20863-img0003.jpg "Immunohistochemistry-Paraffin: Vitamin D BP Antibody [NBP2-20863]")
Immunohistochemistry-Paraffin: Vitamin D BP Antibody [NBP2-20863]
Immunohistochemistry-Paraffin: Vitamin D BP Antibody [NBP2-20863] - Cal27 xenograft, using Vitamin D Binding Protein antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.Applications for Vitamin D BP Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Vitamin D BP
Long Name
Vitamin D Binding Protein
Alternate Names
DBP, DBP-MAF, Gc, Gc-MAF, VDBG, VDBP
Gene Symbol
GC
Additional Vitamin D BP Products
Product Documents for Vitamin D BP Antibody
Product Specific Notices for Vitamin D BP Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.