Von Willebrand Factor Antibody (VWF/1859R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08963
Recombinant Monoclonal Antibody
![Western Blot: Von Willebrand Factor Antibody (VWF/1859R)Azide and BSA Free [NBP3-08963]](https://resources.bio-techne.com/images/products/vWF-A2-Antibody-VWF-1859R-Azide-and-BSA-Free-Western-Blot-NBP3-08963-img0002.jpg "Western Blot: Von Willebrand Factor Antibody (VWF/1859R)Azide and BSA Free [NBP3-08963]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # VWF/1859R
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for Von Willebrand Factor Antibody (VWF/1859R) - Azide and BSA Free
Immunogen
Recombinant fragment of human Von Willebrand Factor protein (aa1815-1939) (exact sequence is proprietary) (Uniprot: P04275)
Localization
Cytoplasmic
Specificity
von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposis sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.
Marker
Endothelial Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
250 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of recombinant monoclonal antibody purified by Protein A. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07217).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Von Willebrand Factor Antibody (VWF/1859R) - Azide and BSA Free
Western Blot: Von Willebrand Factor Antibody (VWF/1859R)Azide and BSA Free [NBP3-08963]
Western Blot: vWF-A2 Antibody (VWF/1859R) - Azide and BSA Free [NBP3-08963] - Western Blot Analysis A) Recombinant vWF-A2 Protein (B) human lung lysate using vWF-A2 antibody (VWF/1859R).![Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody (VWF/1859R) - Azide and BSA Free [NBP3-08963]](https://resources.bio-techne.com/images/products/vWF-A2-Antibody-VWF-1859R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08963-img0001.jpg "Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody (VWF/1859R) - Azide and BSA Free [NBP3-08963]")
Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody (VWF/1859R) - Azide and BSA Free [NBP3-08963]
Immunohistochemistry-Paraffin: vWF-A2 Antibody (VWF/1859R) - Azide and BSA Free [NBP3-08963] - Formalin-fixed, paraffin-embedded human Tonsil stained with vWF-A2 Rabbit Recombinant Monoclonal Antibody (VWF/1859R).Applications for Von Willebrand Factor Antibody (VWF/1859R) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Von Willebrand Factor
Alternate Names
coagulation factor VIII VWF, F8, F8VWF, von Willebrand factor, VWD, vWF
Gene Symbol
VWF
Additional Von Willebrand Factor Products
Product Documents for Von Willebrand Factor Antibody (VWF/1859R) - Azide and BSA Free
Product Specific Notices for Von Willebrand Factor Antibody (VWF/1859R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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