Von Willebrand Factor Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB600-586
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse, Canine, Feline
Cited:
Human, Mouse, Canine, Feline
Applications
Validated:
Dual RNAscope ISH-IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Purified antisera of the Von Willebrand Factor (factor VIII R:ag) related antigen in plasma.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 31738896). Feline reactivity reported in scientific literature (PMID:31616579).
Localization
Secreted. Localized to storage granules.
Specificity
The antibody specifically reacts with the cytoplasm of human endothelial cells from normal and neoplastic blood and from lymphatic vessels. It also reacts with human endocardium, platelets and megakaryocytes. It may be used for immunohistological studies of the vascular structures in frozen tissue preparations and enzymatically pre-digested, formalin, methacarn, Bouin's or ethanol-fixed and paraffin-embedded tissue sections.
Marker
Endothelial Cell Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Von Willebrand Factor Antibody
Dual RNAscope ISH-IHC: Von Willebrand Factor Antibody [NB600-586] - Formalin-fixed paraffin-embedded tissue sections of human metastatic tonsil were probed for vWF mRNA (ACD RNAScope probe, catalog # 5860461; Fast Red chromogen, ACD catalog # 322500). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal (Novus catalog # NB600-586) at 1:500 dilution for 1 hour at room temperature followed by incubation with the anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue).
Immunohistochemistry: Von Willebrand Factor Antibody [NB600-586]
Immunohistochemistry: Von Willebrand Factor Antibody [NB600-586] - Formalin fixed paraffin embedded human tonsil stained with Factor VIII antibody.Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody [NB600-586]
Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody [NB600-586] - Staining of formalin-fixed, paraffin-embedded Human Kidney sections with 1:2,000 Rabbit Anti-von Willebrand Factor.Applications for Von Willebrand Factor Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:200
Immunohistochemistry
1:1000
Immunohistochemistry-Paraffin
1:1000
Application Notes
IHC-P: We recommend proteolycic antigen retrieval. After deparaffinization treat with 0.1% Pronase in PBS for 5-10 minutes at 37 degrees C. Alternatively, digest with 0.1% Trypsin in PBS for 25 minutes at 37 degrees C. Gently rinse in PBS. This Von Willebrand Factor Antibody is validated for IHC-Fr from a verified customer review.
Reviewed Applications
Read 3 reviews rated 4.3 using NB600-586 in the following applications:
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
10 mM PBS (pH 7.4)
Preservative
0.9% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Von Willebrand Factor
Alternate Names
coagulation factor VIII VWF, F8, F8VWF, von Willebrand factor, VWD, vWF
Entrez Gene IDs
7450 (Human)
Gene Symbol
VWF
UniProt
Additional Von Willebrand Factor Products
Product Documents for Von Willebrand Factor Antibody
Product Specific Notices for Von Willebrand Factor Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...