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VprBP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-49073

Novus Biologicals, part of Bio-Techne
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NBP2-49073
NBP2-49073-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: IAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VprBP Antibody

Immunocytochemistry/ Immunofluorescence: VprBP Antibody [NBP2-49073]

Immunocytochemistry/ Immunofluorescence: VprBP Antibody [NBP2-49073]

Immunocytochemistry/Immunofluorescence: VprBP Antibody [NBP2-49073] - Staining of human cell line CACO-2 shows localization to nucleus & nucleoli fibrillar center.
Immunohistochemistry-Paraffin: VprBP Antibody [NBP2-49073]

Immunohistochemistry-Paraffin: VprBP Antibody [NBP2-49073]

Immunohistochemistry-Paraffin: VprBP Antibody [NBP2-49073] - Staining of human kidney shows strong cytoplasmic positivity in renal tubules.

Applications for VprBP Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VprBP

VprBP (HIV-1 Vpr-binding protein) is also known as DCAF1 (Ddb1- and Cul4-associated factor 1). VprBP was initially identified as a protein that co-immunoprecipitated with the HIV protein, viral protein R; however the physiological relevance of this association remains unclear. It has recently been shown that VprBP plays an essential role in cell cycle progression, and it associates with the DDB1-CUL4 E3 ligase complex. Alternate names for VprBP include Vpr-interacting protein, RIP, and KIAA0800.

Alternate Names

DCAF1DDB1 and CUL4 associated factor 1, DDB1- and CUL4-associated factor 1, HIV-1 Vpr-binding protein, KIAA0800protein VPRBP, MGC102804, RIP, Vpr (HIV-1) binding protein, Vpr-binding protein, VprBP, Vpr-interacting protein

Gene Symbol

VPRBP

Additional VprBP Products

Product Documents for VprBP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VprBP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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