VPS25 Antibody (2E5-2B9)
Novus Biologicals, part of Bio-Techne | Catalog # H00084313-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2E5-2B9
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
MGC10540 (AAH06282, 1 a.a. ~ 176 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAMSFEWPWQYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLHKQSSMTVMEAQESPLFNNVKLQRKLPVESIQIVLEELRKKGNLEWLDKSKSSFLIMWRRPEEWGKLIYQWVSRSGQNNSVFTLYELTNGEDTEDEEFHGLDEATLLRALQALQQEHKAEIITVSDGRGVKFF
Reactivity Notes
Human. Other species not tested.
Specificity
VPS25 - hypothetical protein MGC10540
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for VPS25 Antibody (2E5-2B9)
![Western Blot: VPS25 Antibody (2E5-2B9) [H00084313-M01]](https://resources.bio-techne.com/images/products/VPS25-Antibody-2E5-2B9-Western-Blot-H00084313-M01-img0007.jpg "Western Blot: VPS25 Antibody (2E5-2B9) [H00084313-M01]")
Western Blot: VPS25 Antibody (2E5-2B9) [H00084313-M01]
Western Blot: VPS25 Antibody (2E5-2B9) [H00084313-M01] - MGC10540 monoclonal antibody (M01), clone 2E5-2B9 Analysis of MGC10540 expression in Hela S3 NE.![Immunocytochemistry/ Immunofluorescence: VPS25 Antibody (2E5-2B9) [H00084313-M01]](https://resources.bio-techne.com/images/products/VPS25-Antibody-2E5-2B9-Immunocytochemistry-Immunofluorescence-H00084313-M01-img0005.jpg "Immunocytochemistry/ Immunofluorescence: VPS25 Antibody (2E5-2B9) [H00084313-M01]")
Immunocytochemistry/ Immunofluorescence: VPS25 Antibody (2E5-2B9) [H00084313-M01]
Immunocytochemistry/Immunofluorescence: VPS25 Antibody (2E5-2B9) [H00084313-M01] - Analysis of monoclonal antibody to VPS25 on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: VPS25 Antibody (2E5-2B9) [H00084313-M01] - Detection limit for recombinant GST tagged VPS25 is approximately 0.03ng/ml as a capture antibody.
Applications for VPS25 Antibody (2E5-2B9)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: VPS25
Alternate Names
Dermal papilla-derived protein 9, DERP9, DERP9hVps25, EAP20, EAP20FAP20, ELL-associated protein of 20 kDa, ESCRT-II complex subunit VPS25, FAP20, MGC10540, vacuolar protein sorting 25 (yeast), vacuolar protein sorting 25 homolog (S. cerevisiae), vacuolar protein-sorting-associated protein 25
Entrez Gene IDs
84313 (Human)
Gene Symbol
VPS25
UniProt
Additional VPS25 Products
Product Documents for VPS25 Antibody (2E5-2B9)
Product Specific Notices for VPS25 Antibody (2E5-2B9)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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