VPS26A Antibody (CL2287)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36754

Novus Biologicals, part of Bio-Techne
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NBP2-36754
NBP2-36754-25ul
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (96%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2287

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all VPS26A Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids:LRKQRTNFHQRFESPESQASAEQPEM

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for VPS26A Antibody (CL2287)

Immunohistochemistry-Paraffin: VPS26A Antibody (CL2287) [NBP2-36754]

Immunohistochemistry-Paraffin: VPS26A Antibody (CL2287) [NBP2-36754]

Immunohistochemistry-Paraffin: VPS26A Antibody (CL2287) [NBP2-36754] - Staining in human testis and skeletal muscle tissues. Corresponding VPS26A RNA-seq data are presented for the same tissues.
Western Blot: VPS26A Antibody (CL2287) [NBP2-36754]

Western Blot: VPS26A Antibody (CL2287) [NBP2-36754]

Western Blot: VPS26A Antibody (CL2287) [NBP2-36754] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-VPS26A monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % inten
Immunocytochemistry/ Immunofluorescence: VPS26A Antibody (CL2287) [NBP2-36754]

Immunocytochemistry/ Immunofluorescence: VPS26A Antibody (CL2287) [NBP2-36754]

Immunocytochemistry/Immunofluorescence: VPS26A Antibody (CL2287) [NBP2-36754] - Staining in U251 cell line showing distinct staining of endosomes in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
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Applications for VPS26A Antibody (CL2287)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VPS26A

VPS26 belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.

Alternate Names

FLJ12930, HB58, Hbeta58, hVPS26, PEP8A, vacuolar protein sorting 26 (yeast homolog), vacuolar protein sorting 26 homolog A (S. pombe), vacuolar protein sorting 26 homolog A (yeast), vacuolar protein sorting-associated protein 26A, Vesicle protein sorting 26A, VPS26vacuolar protein sorting 26 (yeast)

Gene Symbol

VPS26A

UniProt

O75436 (Human)

Additional VPS26A Products

Product Documents for VPS26A Antibody (CL2287)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for VPS26A Antibody (CL2287)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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