VPS26A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-49257

Novus Biologicals, part of Bio-Techne
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NBP2-49257
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all VPS26A Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LRKQRTNFHQRFESPESQASAEQPEM

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VPS26A Antibody

Western Blot: VPS26A Antibody [NBP2-49257]

Western Blot: VPS26A Antibody [NBP2-49257]

Western Blot: VPS26 Antibody [NBP2-49257] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251 MG
Lane 4: Human plasma
Lane 5: Human Liver tissue
Lane 6: Human Tonsil tissue
Immunocytochemistry/ Immunofluorescence: VPS26A Antibody [NBP2-49257]

Immunocytochemistry/ Immunofluorescence: VPS26A Antibody [NBP2-49257]

Immunocytochemistry/Immunofluorescence: VPS26 Antibody [NBP2-49257] - Staining of human cell line U-2 OS shows localization to endosomes & lysosomes. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: VPS26A Antibody [NBP2-49257]

Immunohistochemistry-Paraffin: VPS26A Antibody [NBP2-49257]

Immunohistochemistry-Paraffin: VPS26 Antibody [NBP2-49257] - Staining of human testis shows high expression.
View 2 more images

Applications for VPS26A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VPS26A

VPS26 belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.

Alternate Names

FLJ12930, HB58, Hbeta58, hVPS26, PEP8A, vacuolar protein sorting 26 (yeast homolog), vacuolar protein sorting 26 homolog A (S. pombe), vacuolar protein sorting 26 homolog A (yeast), vacuolar protein sorting-associated protein 26A, Vesicle protein sorting 26A, VPS26vacuolar protein sorting 26 (yeast)

Gene Symbol

VPS26A

Additional VPS26A Products

Product Documents for VPS26A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for VPS26A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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