VPS26A Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75922

Novus Biologicals, part of Bio-Techne
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NBP2-75922
NBP2-75922SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all VPS26A Antibodies »

Product Specifications

Immunogen

Partial synthetic peptide from the C-terminal of human VPS26A (between amino acids 250-320) [UniProt O75436]

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VPS26A Antibody - BSA Free

Western Blot: VPS26A AntibodyBSA Free [NBP2-75922]

Western Blot: VPS26A AntibodyBSA Free [NBP2-75922]

Western Blot: VPS26 Antibody [NBP2-75922] - Total protein from A431, HeLa, and HepG2 was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-VPS26A in 2.5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunocytochemistry/ Immunofluorescence: VPS26A Antibody - BSA Free [NBP2-75922]

Immunocytochemistry/ Immunofluorescence: VPS26A Antibody - BSA Free [NBP2-75922]

Immunocytochemistry/Immunofluorescence: VPS26A Antibody [NBP2-75922] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-VPS26A Antibody NBP2-75922 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunohistochemistry-Paraffin: VPS26A Antibody - BSA Free [NBP2-75922]

Immunohistochemistry-Paraffin: VPS26A Antibody - BSA Free [NBP2-75922]

Immunohistochemistry-Paraffin: VPS26 Antibody [NBP2-75922] - IHC analysis of a formalin fixed paraffin embedded tissue section of the mouse testis using 1:350 dilution of VPS26A antibody (NBP2-75922). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin.

Applications for VPS26A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-5 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

2 ug/ml

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VPS26A

VPS26A, also known as vacuolar protein sorting-associated protein 26A, is essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also this protein is required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Recombinant human VPS26A protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

FLJ12930, HB58, Hbeta58, hVPS26, PEP8A, vacuolar protein sorting 26 (yeast homolog), vacuolar protein sorting 26 homolog A (S. pombe), vacuolar protein sorting 26 homolog A (yeast), vacuolar protein sorting-associated protein 26A, Vesicle protein sorting 26A, VPS26vacuolar protein sorting 26 (yeast)

Gene Symbol

VPS26A

UniProt

O75436 (Human)

Additional VPS26A Products

Product Documents for VPS26A Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for VPS26A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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