VPS28 Antibody (4F8M0)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15703

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15703-100ul
NBP3-15703-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4F8M0

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all VPS28 Antibodies »

Product Summary for VPS28 Antibody (4F8M0)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 122-221 of human VPS28 (Q9UK41). KGNLNRCIADVVSLFITVMDKLRLEIRAMDEIQPDLRELMETMHRMSHLPPDFEGRQTVSQWLQTLSGMSASDELDDSQVRQMLFDLESAYNAFNRFLHA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VPS28 Antibody (4F8M0)

Western Blot: VPS28 Antibody (4F8M0) [NBP3-15703]

Western Blot: VPS28 Antibody (4F8M0) [NBP3-15703]

Western Blot: VPS28 Antibody (4F8M0) [NBP3-15703] - Western blot analysis of extracts of various cell lines, using VPS28 antibody (NBP3-15703) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: VPS28 Antibody (4F8M0) [NBP3-15703]

Immunohistochemistry-Paraffin: VPS28 Antibody (4F8M0) [NBP3-15703]

Immunohistochemistry-Paraffin: VPS28 Antibody (4F8M0) [NBP3-15703] - Immunohistochemistry of paraffin-embedded human lung cancer using VPS28 Rabbit mAb (NBP3-15703) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for VPS28 Antibody (4F8M0)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VPS28

VPS28 encodes a protein involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. The encoded protein is one of the three subunits of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. The two other subunits of ESCRT-I are vesicular protein sorting 23, also known as tumor susceptibility gene 101 (TSG101), and vesicular protein sorting 37. Two alternative transcripts encoding different isoforms have been described. Additional alternative transcripts may exist but the proteins encoded by these transcripts have not been verified experimentally.

Alternate Names

ESCRT-I complex subunit VPS28, H-Vps28, MGC60323, vacuolar protein sorting 28 (yeast), vacuolar protein sorting 28 homolog (S. cerevisiae), vacuolar protein sorting-associated protein 28 homolog, yeast class E protein Vps28p homolog

Gene Symbol

VPS28

Additional VPS28 Products

Product Documents for VPS28 Antibody (4F8M0)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for VPS28 Antibody (4F8M0)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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