Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1A8

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all VPS28 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human VPS28(NP_057292) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

25.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VPS28 Antibody (OTI1A8) - Azide and BSA Free

Western Blot: VPS28 Antibody (OTI1A8)Azide and BSA Free [NBP2-74854]

Western Blot: VPS28 Antibody (OTI1A8)Azide and BSA Free [NBP2-74854]

Western Blot: VPS28 Antibody (OTI1A8) - Azide and BSA Free [NBP2-74854] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VPS28.
Immunocytochemistry/ Immunofluorescence: VPS28 Antibody (OTI1A8) - Azide and BSA Free [NBP2-74854]

Immunocytochemistry/ Immunofluorescence: VPS28 Antibody (OTI1A8) - Azide and BSA Free [NBP2-74854]

Immunocytochemistry/Immunofluorescence: VPS28 Antibody (OTI1A8) - Azide and BSA Free [NBP2-74854] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY VPS28.
Immunohistochemistry: VPS28 Antibody (OTI1A8) - Azide and BSA Free [NBP2-74854]

Immunohistochemistry: VPS28 Antibody (OTI1A8) - Azide and BSA Free [NBP2-74854]

Immunohistochemistry: VPS28 Antibody (OTI1A8) - Azide and BSA Free [NBP2-74854] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Applications for VPS28 Antibody (OTI1A8) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:200-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VPS28

VPS28 encodes a protein involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. The encoded protein is one of the three subunits of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. The two other subunits of ESCRT-I are vesicular protein sorting 23, also known as tumor susceptibility gene 101 (TSG101), and vesicular protein sorting 37. Two alternative transcripts encoding different isoforms have been described. Additional alternative transcripts may exist but the proteins encoded by these transcripts have not been verified experimentally.

Alternate Names

ESCRT-I complex subunit VPS28, H-Vps28, MGC60323, vacuolar protein sorting 28 (yeast), vacuolar protein sorting 28 homolog (S. cerevisiae), vacuolar protein sorting-associated protein 28 homolog, yeast class E protein Vps28p homolog

Gene Symbol

VPS28

Additional VPS28 Products

Product Documents for VPS28 Antibody (OTI1A8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for VPS28 Antibody (OTI1A8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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