VPS28 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1399

Novus Biologicals, part of Bio-Techne
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NB100-1399
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all VPS28 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-ESAYNAFNRFLHA corresponding to C-Terminus according to NP_057292.1.

Specificity

This antibody is expected to recognize isoform 1 (NP_057292.1) only.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for VPS28 Antibody

Western Blot: VPS28 Antibody [NB100-1399]

Western Blot: VPS28 Antibody [NB100-1399]

Western Blot: VPS28 Antibody [NB100-1399] - (1ug/ml) of Human Testis lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry-Paraffin: VPS28 Antibody [NB100-1399]

Immunohistochemistry-Paraffin: VPS28 Antibody [NB100-1399]

Immunohistochemistry-Paraffin: VPS28 Antibody [NB100-1399] - (10ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Applications for VPS28 Antibody

Application
Recommended Usage

Immunohistochemistry

10 ug/ml

Immunohistochemistry-Paraffin

10 ug/ml

Peptide ELISA

Detection limit 1:1000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 24 kDa band observed in H460, human testis and brain lysates (predicted MW of 25 kDa band according to NP_057292.1). IHC-P: Human testis stains acrosomal head caps in round spermatids.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VPS28

This gene encodes a protein involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. The encoded protein is one of the three subunits of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. The two other subunits of ESCRT-I are vesicular protein sorting 23, also known as tumor susceptibility gene 101 (TSG101), and vesicular protein sorting 37. Two alternative transcripts encoding different isoforms have been described. Additional alternative transcripts may exist but the proteins encoded by these transcripts have not been verified experimentally.

Alternate Names

ESCRT-I complex subunit VPS28, H-Vps28, MGC60323, vacuolar protein sorting 28 (yeast), vacuolar protein sorting 28 homolog (S. cerevisiae), vacuolar protein sorting-associated protein 28 homolog, yeast class E protein Vps28p homolog

Entrez Gene IDs

51160 (Human); 66914 (Mouse)

Gene Symbol

VPS28

UniProt

NP_057292. (Human)

Additional VPS28 Products

Product Documents for VPS28 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for VPS28 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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