VPS33B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20874

Novus Biologicals, part of Bio-Techne
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NBP2-20874
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (98%), Chicken (90%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all VPS33B Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human VPS33B. The exact sequence is proprietary.

Localization

Late endosome membrane; Peripheral membrane protein; Cytoplasmic side; Lysosome membrane; Peripheral membrane protein; Cytoplasmic side

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VPS33B Antibody

Immunohistochemistry-Paraffin: VPS33B Antibody [NBP2-20874]

Immunohistochemistry-Paraffin: VPS33B Antibody [NBP2-20874]

Immunohistochemistry-Paraffin: VPS33B Antibody [NBP2-20874] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.
VPS33B Antibody

Western Blot: VPS33B Antibody [NBP2-20874] -

Western Blot: VPS33B Antibody [NBP2-20874] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS33B antibody [N3C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
VPS33B Antibody

Western Blot: VPS33B Antibody [NBP2-20874] -

Western Blot: VPS33B Antibody [NBP2-20874] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS33B antibody [N3C2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Applications for VPS33B Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: VPS33B

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec-1 domain family, and encodes the human ortholog of rat Vps33b which is homologous to the yeast class C Vps33 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. [provided by RefSeq]

Alternate Names

FLJ14848, hVPS33B, vacuolar protein sorting 33 homolog B (yeast), vacuolar protein sorting 33B, vacuolar protein sorting 33B (yeast homolog), vacuolar protein sorting-associated protein 33B

Gene Symbol

VPS33B

UniProt

Q9H267 (Human)

Additional VPS33B Products

Product Documents for VPS33B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for VPS33B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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