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VPS34 Antibody (HL2305) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25745

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25745

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat, Canine

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2305

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fragmemt of human VPS34.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VPS34 Antibody (HL2305) - Azide and BSA Free

VPS34 Antibody (HL2305) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: VPS34 Antibody (HL2305) - Azide and BSA Free [NBP3-25745] -

Immunocytochemistry/Immunofluorescence: VPS34 Antibody (HL2305) - Azide and BSA Free [NBP3-25745] - VPS34 antibody [HL2305] detects VPS34 protein at endoplasmic reticulum and cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS34 stained by VPS34 antibody [HL2305] (NBP3-25745) diluted at 1:500. Blue: Fluoroshield with DAPI .
VPS34 Antibody (HL2305) - Azide and BSA Free

Western Blot: VPS34 Antibody (HL2305) - Azide and BSA Free [NBP3-25745] -

Western Blot: VPS34 Antibody (HL2305) - Azide and BSA Free [NBP3-25745] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS34 antibody [HL2305] (NBP3-25745) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
VPS34 Antibody (HL2305) - Azide and BSA Free

Western Blot: VPS34 Antibody (HL2305) - Azide and BSA Free [NBP3-25745] -

Western Blot: VPS34 Antibody (HL2305) - Azide and BSA Free [NBP3-25745] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS34 antibody [HL2305] (NBP3-25745) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for VPS34 Antibody (HL2305) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VPS34/PIK3C3

VPS34, also known as phosphoinositide-3-kinase p100 subunit or PIK3C3 (phosphoinositide-3-kinase, class III), is a member of the PI3/PI4-kinase family. It is ubiquitously expressed with predominant expression in skeletal muscle and is believed to participate in endosome to lysosome transport, multivesicular body formation, autophagy and retrograde endosome to Golgi transport. VPS34 is the catalytic subunit of class III PI3Ks and forms a heterodimer with p150, a regulatory subunit of class 3 PI3Ks. It exclusively phosphorylates phosphatidylinositol to produce PtdIns3P. The activity of VPS34 is enhanced by manganese and can be regulated by nutrients, suggesting an important role of VPS34 in the regulation of mTOR protein synthesis. VPS34 also forms a complex with Beclin1 that promotes autophagy and tumor supression.

Alternate Names

PI 3-Kinase C3, PI3K type 3, PIK3C3

Gene Symbol

PIK3C3

Additional VPS34/PIK3C3 Products

Product Documents for VPS34 Antibody (HL2305) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VPS34 Antibody (HL2305) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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