VPS35 Antibody (8A3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-78825
![Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825]](https://resources.bio-techne.com/images/products/VPS35-Antibody-8A3-Immunoprecipitation-NBP2-78825-img0001.jpg "Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 8A3
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for VPS35 Antibody (8A3)
Immunogen
Full length recombinant human VSP35
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for VPS35 Antibody (8A3)
![Western Blot: VPS35 Antibody (8A3) [NBP2-78825]](https://resources.bio-techne.com/images/products/VPS35-Antibody-8A3-Western-Blot-NBP2-78825-img0012.jpg "Western Blot: VPS35 Antibody (8A3) [NBP2-78825]")
Western Blot: VPS35 Antibody (8A3) [NBP2-78825]
Western Blot: VPS35 Antibody (8A3) [NBP2-78825] - Western Blot analysis of Human SH-SY5Y showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (NBP2-78825). Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.![Immunohistochemistry: VPS35 Antibody (8A3) [NBP2-78825]](https://resources.bio-techne.com/images/products/VPS35-Antibody-8A3-Immunohistochemistry-NBP2-78825-img0013.jpg "Immunohistochemistry: VPS35 Antibody (8A3) [NBP2-78825]")
Immunohistochemistry: VPS35 Antibody (8A3) [NBP2-78825]
Immunohistochemistry: VPS35 Antibody (8A3) [NBP2-78825] - Immunohistochemistry analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (NBP2-78825). Tissue: Kidney. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 20X.![Western Blot: VPS35 Antibody (8A3) [NBP2-78825]](https://resources.bio-techne.com/images/products/VPS35-Antibody-8A3-Western-Blot-NBP2-78825-img0011.jpg "Western Blot: VPS35 Antibody (8A3) [NBP2-78825]")
Western Blot: VPS35 Antibody (8A3) [NBP2-78825]
Western Blot: VPS35 Antibody (8A3) [NBP2-78825] - Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (NBP2-78825). Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825) at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.![Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825]](https://resources.bio-techne.com/images/products/VPS35-Antibody-8A3-Immunoprecipitation-NBP2-78825-img0008.jpg "Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825]")
![Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825]](https://resources.bio-techne.com/images/products/VPS35-Antibody-8A3-Immunoprecipitation-NBP2-78825-img0009.jpg "Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825]")
![Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825]](https://resources.bio-techne.com/images/products/VPS35-Antibody-8A3-Immunoprecipitation-NBP2-78825-img0010.jpg "Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825]")
Applications for VPS35 Antibody (8A3)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:200
Immunohistochemistry
1:100
Immunoprecipitation
1:200
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: VPS35
Alternate Names
DKFZp434P1672, FLJ10752, FLJ13588, FLJ20388, hVPS35, Maternal-embryonic 3, MEM3DKFZp434E1211, vacuolar protein sorting 35 (yeast homolog), vacuolar protein sorting 35 (yeast), vacuolar protein sorting 35 homolog (S. cerevisiae), vacuolar protein sorting-associated protein 35, Vesicle protein sorting 35
Gene Symbol
VPS35
Additional VPS35 Products
Product Documents for VPS35 Antibody (8A3)
Product Specific Notices for VPS35 Antibody (8A3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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