VPS45 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2431

Novus Biologicals, part of Bio-Techne
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NB100-2431
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all VPS45 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-FQKKKPKEQQKLES corresponding to internal region according to NP_009190.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for VPS45 Antibody

Western Blot: VPS45 Antibody [NB100-2431]

Western Blot: VPS45 Antibody [NB100-2431]

Western Blot: VPS45 Antibody [NB100-2431] - (0.03ug/ml) staining of human placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-2431]

Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-2431]

Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-2431] - Staining of Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.taining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for VPS45 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

2.5 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.03 - 0.1 ug/ml
Application Notes
WB: Approx. 55 kDa band observed in human placenta lysates (calculated MW of 65.1 kDa band according to NP_009190.2).
IHC: Paraffin embedded Human Spleen.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VPS45

Vesicle mediated protein sorting is important for the distribution of intracellular molecules into distinct organelles. More than 40 vacuolar protein sorting (VPS) genes are involved in this process. VPS45A is a member of the Sec1 domain family. Its precise function unknown, but its high expression in peripheral blood mononuclear cells suggests a role in trafficking proteins, including inflammatory mediators.

Alternate Names

H1, H1VPS45, hlVps45, h-vps45, leucocyte vacuolar protein sorting 45, vacuolar protein sorting 45 homolog (S. cerevisiae), vacuolar protein sorting 45A, vacuolar protein sorting 45A (yeast homolog), vacuolar protein sorting 45A (yeast), vacuolar protein sorting-associated protein 45, VPS45A, VPS45AVPS54A, VPS45B, VSP45, VSP45A

Entrez Gene IDs

11311 (Human); 22365 (Mouse); 64516 (Rat)

Gene Symbol

VPS45

UniProt

NP_009190.2 (Human)

Additional VPS45 Products

Product Documents for VPS45 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for VPS45 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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