Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all VPS4B Antibodies »

Product Summary for VPS4B Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 300 and 350 of human vacuolar protein sorting 4 homolog B using the numbering given in entry NP_004860.2 (GeneID 9525).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VPS4B Antibody

Immunoprecipitation: VPS4B Antibody [NBP1-19118]

Immunoprecipitation: VPS4B Antibody [NBP1-19118]

Immunoprecipitation: VPS4B Antibody [NBP1-19118] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-VPS4B antibody used for IP at 3 mcg/mg lysate. VPS4B was also immunoprecipitated by rabbit anti- VPS4B antibody NBP1-19119, which recognizes a downstream epitope

Applications for VPS4B Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
It is not recommended for western analysis.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: VPS4B

The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16. [provided by RefSeq]

Alternate Names

Cell migration-inducing gene 1 protein, Protein SKD1, SKD1B, SKD1cell migration-inducing 1, Suppressor of K(+) transport growth defect 1, suppressor of K+ transport defect 1, vacuolar protein sorting 4 homolog B (S. cerevisiae), vacuolar protein sorting 4B, vacuolar protein sorting 4B (yeast), vacuolar protein sorting-associated protein 4B, VPS42, VPS4-2

Entrez Gene IDs

9525 (Human)

Gene Symbol

VPS4B

UniProt

NP_004860.2 (Human)

Additional VPS4B Products

Product Documents for VPS4B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VPS4B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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