VRL1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92576

Novus Biologicals, part of Bio-Techne
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NBP1-92576
NBP1-92576-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all VRL1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RGKLDFGSGLPPMESQFQGEDRKFAPQIRVNLNYRKGTGASQPDPNRFDRDRLFNAVSRGVPEDLAGLPEYLSKTSKYLTDS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VRL1 Antibody

Immunocytochemistry/ Immunofluorescence: VRL1 Antibody [NBP1-92576]

Immunocytochemistry/ Immunofluorescence: VRL1 Antibody [NBP1-92576]

Immunocytochemistry/Immunofluorescence: VRL1 Antibody [NBP1-92576] - Staining of human cell line SK-MEL-30 shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: VRL1 Antibody [NBP1-92576]

Immunohistochemistry-Paraffin: VRL1 Antibody [NBP1-92576]

Immunohistochemistry-Paraffin: VRL1 Antibody [NBP1-92576] - Staining of human gastrointestinal shows strong cytoplasmic positivity in peripheral ganglion.
Immunohistochemistry-Paraffin: VRL1 Antibody [NBP1-92576]

Immunohistochemistry-Paraffin: VRL1 Antibody [NBP1-92576]

Immunohistochemistry-Paraffin: VRL1 Antibody [NBP1-92576] - Staining of human liver shows no positivity in hepatocytes as expected.
View 2 more images

Applications for VRL1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VRL1

Caterina et al has identified a structurally related receptor, VRL-1, that does not respond to capsaicin, acid or moderate heat. Instead, VRL-1 is activated by high temperatures, with a threshold of approximately 52 degrees C. Within sensory ganglia, VRL-1 is most prominently expressed by a subset of medium- to large-diameter neurons, making it a candidate receptor for transducing high-threshold heat responses in this class of cells. VRL-1 transcripts are not restricted to the sensory nervous system, indicating that this channel may be activated by stimuli other than heat. VRL-1 is one of the six members of the vanilloid-like TRP-channel family which is now known as TRPV family. FUNCTION: Calcium-permeable, non-selective cation channel with an outward rectification. Seems to be regulated, at least in part, by growth factors, like IGF1, PDGF and morphogenetic neuropeptide/head activator. May transduce physical stimuli in mast cells. Activated by temperatures higher than 52 degrees Celsius; is not activated by vanilloids and acidic pH. SUBUNIT: Homotetramer (Probable). Interacts with a cAMP-dependent protein kinase type II regulatory subunit (PRKAR2A or PRKAR2B) and ACBD3. Interacts with RGA. SUBCELLULAR LOCATION: Cell membrane; multi-pass membrane protein. Cytoplasm. Translocates from the cytoplasm to the plasma membrane upon ligand stimulation. TISSUE SPECIFICITY: Ubiquitously expressed. Expressed in dorsal root ganglia, trigeminal ganglia, spinal chord (Lissauer's tract, dorsal horn and dorsal columns) (at protein level). PTM: N-glycosylated. PTM: Phosphorylated by PKA. SIMILARITY: Belongs to the transient receptor family. TrpV subfamily. SIMILARITY: Contains 3 ANK repeats.

Long Name

Transient receptor potential cation channel subfamily V member 2

Alternate Names

OTRPC2, TrpV2, VRL, VRL-1

Gene Symbol

TRPV2

Additional VRL1 Products

Product Documents for VRL1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for VRL1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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