WASF1/WAVE1 [p Tyr125] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23190

Novus Biologicals, part of Bio-Techne
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NBP3-23190
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all WASF1/WAVE1 Antibodies »

Product Specifications

Immunogen

Phospho-WASF1/WAVE1 (Tyr-125) peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-125 in human WASF1/WAVE1. This sequence has high homology with similar regions in rat and mouse WASF1/WAVE1, and has less than 50% homology to similar regions in the conserved site in WAVE2 (Tyr-124) and WAVE3 (Tyr-125). [UniProt# Q92558]

Modification

p Tyr125

Specificity

This antibody was cross-adsorbed to phospho-WASF1/WAVE1 (Tyr-150) and unphosphorylated WASF1/WAVE1 (Tyr-125) peptides before affinity purification using phospho-WASF1/WAVE1 (Tyr-125) peptide. In western blots, the antibody detects an 80 kDa band corresponding to phosphorylated WASF1/WAVE1 in human SYF cSrc transformed cells and mouse macrophages treated with pervanadate, but is not observed in control cells. These bands can be specifically blocked with phospho-WASF1/WAVE1 (Tyr-125) peptide (WX1775), but not phospho-WASF1/WAVE1 (Tyr-150) peptide.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for WASF1/WAVE1 [p Tyr125] Antibody

WASF1/WAVE1 [p Tyr125] Antibody

Immunocytochemistry/Immunofluorescence: WASF1/WAVE1 [p Tyr125] Antibody [NBP3-23190] -

Immunocytochemistry/Immunofluorescence: WASF1/WAVE1 [p Tyr125] Antibody [NBP3-23190] - Immunocytochemical labeling of phosphorylated WAVE in pervanadate-treated mouse C2C12. The cells were labeled with rabbit polyclonal WASF1/WAVE1 (N-terminal region) and WAVE (Tyr-125) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
WASF1/WAVE1 [p Tyr125] Antibody

Western Blot: WASF1/WAVE1 [p Tyr125] Antibody [NBP3-23190] -

Western Blot: WASF1/WAVE1 [p Tyr125] Antibody [NBP3-23190] - Western blot of human SYF cSrc transformed cells untreated (lanes 1 & 3) or treated (lanes 2 & 4) with pervanadate (1 mM; 30 min). The blots were probed with anti-WASF1/WAVE1 (N-terminal region) (lanes 1 & 2) or anti-WAVE (Tyr-125) (lanes 3 & 4).

Applications for WASF1/WAVE1 [p Tyr125] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: WASF1/WAVE1

WASP (for Wiskott-Aldrich syndrome protein), N-WASP are downstream effectors of Cdc42 that are implicated in actin polymerization and cytoskeletal organization. The WASP family also includes VASP (vasodilator-stimulated phosphoprotein) and Mena (for mammalian enabled protein), which accumulate at focal adhesions and are also involved in the regulation of the actin cytoskeleton. The WAVE proteins are related to the WASP family proteins and are likewise involved in mediating actin reorganization downstream of the Rho family of small GTPases. The two protein homologs WAVE1 and WAVE2 specifically regulate membrane ruffling by inducing the formation of actin filament clusters in response to GTP binding and activating Rac. The WAVE proteins mediate this actin polymerization by cooperating with the Arp2/3 complex, a nucleation core, and thereby promoting the formation of actin filaments. WAVE1, which is also designated SCAR (for suppressor of cAR), is expressed primarily in the brain, while WAVE2 is widely expressed with the expression highest in peripheral blood leukocytes.

Long Name

Wiskott-Aldrich Syndrome Protein Family Member 1

Alternate Names

SCAR1, WASP1, WAVE1

Gene Symbol

WASF1

Additional WASF1/WAVE1 Products

Product Documents for WASF1/WAVE1 [p Tyr125] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for WASF1/WAVE1 [p Tyr125] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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