WASF1/WAVE1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23363
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminal half of human WASF1/WAVE1. This sequence has high homology with similar regions in rat and mouse WASF1/WAVE1, and has less than 50% homology to similar regions in human WAVE2 and WAVE3. [UniProt# Q92558]
Specificity
The antibody detects an 80 kDa protein corresponding to the molecular mass of WASF1/WAVE1 on SDS-PAGE immunoblots of human SYF cSrc-transformed, HUVEC, and K562 cells, as well as rat PC12 cells and mouse C2C12 cells.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for WASF1/WAVE1 Antibody
Western Blot: WASF1/WAVE1 Antibody [NBP3-23363] -
Western Blot: WASF1/WAVE1 Antibody [NBP3-23363] - Western blot of human SYF cSrc-transformed cells. Blots were were probed with anti-WASF1/WAVE1 (N-terminal region) at a dilution of 1:1000 (lane 1), 1:2000 (lane 2) or 1:4000 (lane 3). In addition, the antibody was used in the absence (lane 4) or presence of blocking peptides, WASF1/WAVE1 (N-terminal region) peptide (lane 5) or WASF2 (Central region) peptide (lane 6).
Immunocytochemistry/Immunofluorescence: WASF1/WAVE1 Antibody [NBP3-23363] -
Immunocytochemistry/Immunofluorescence: WASF1/WAVE1 Antibody [NBP3-23363] - Immunocytochemical labeling of phosphorylated WAVE in pervanadate-treated mouse C2C12. The cells were labeled with rabbit polyclonal WASF1/WAVE1 (N-terminal region) and WAVE (Tyr-125) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.Applications for WASF1/WAVE1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:200
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: WASF1/WAVE1
Long Name
Wiskott-Aldrich Syndrome Protein Family Member 1
Alternate Names
SCAR1, WASP1, WAVE1
Gene Symbol
WASF1
Additional WASF1/WAVE1 Products
Product Documents for WASF1/WAVE1 Antibody
Product Specific Notices for WASF1/WAVE1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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