WASF2 [p Tyr150] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23201
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Phospho-WASF2 (Tyr-150) peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-150 in human WASF2. This sequence has high homology with similar regions in rat and mouse WASF2, and has homology to the conserved sites in WAVE1 (Tyr-151) and WAVE3 (Tyr-151). [UniProt# Q9Y6W5]
Modification
p Tyr150
Specificity
This antibody was cross-adsorbed to phospho-WASF2 (Tyr-125) and unphosphorylated WASF2 (Tyr-150) peptides before affinity purification using phospho-WASF2 (Tyr-150) peptide. In western blots, the antibody detects a 78 kDa corresponding to phosphorylated WASF2 in K562 cells treated with pervanadate and a 80 kDa band corresponding to WAVE1 in HUVEC treated with pervanadate. It also recognizes an unidentified band near 50 kDa in K562 cells.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for WASF2 [p Tyr150] Antibody
Western Blot: WASF2 [p Tyr150] Antibody [NBP3-23201] -
Western Blot: WASF2 [p Tyr150] Antibody [NBP3-23201] - Western blot of human K562 cells treated with pervanadate (1 mM, 30 min) (lanes 1 & 3) then treated with alkaline phosphatase (lanes 2 & 4). The blots were probed with anti-WASF2 (Central region) (lanes 1 & 2) or anti-WAVE (Tyr-150) (lanes 3 & 4).Applications for WASF2 [p Tyr150] Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:200
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: WASF2
Alternate Names
IMD2, Protein WAVE-2, SCAR2suppressor of cyclic-AMP receptor (WASP-family), Verprolin homology domain-containing protein 2, WAS protein family, member 2, WASP family Verprolin-homologous protein 2, WAVE2dJ393P12.2, wiskWASP family protein member 2
Gene Symbol
WASF2
Additional WASF2 Products
Product Documents for WASF2 [p Tyr150] Antibody
Product Specific Notices for WASF2 [p Tyr150] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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