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WASHC1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90464

Novus Biologicals, part of Bio-Techne
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NBP1-90464
NBP1-90464-25ul

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GAPREVVDPSGGWATLLESIRQAGGIGKAKLRSMKERKLEKKKQKEQEQVRATSQGGHLMSDLFNKLVMRHKGISGKGPGAGEGPGGAFARVSDSIPPLPPPQQPQVDEDEDDWES

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WASHC1 Antibody

Western Blot: WASHC1 Antibody [NBP1-90464]

Western Blot: WASHC1 Antibody [NBP1-90464]

Western Blot: WASHC1 Antibody [NBP1-90464] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: WASHC1 Antibody [NBP1-90464]

Immunocytochemistry/ Immunofluorescence: WASHC1 Antibody [NBP1-90464]

Immunocytochemistry/Immunofluorescence: WASHC1 Antibody [NBP1-90464] - Staining of human cell line U-251MG shows positivity in vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: WASHC1 Antibody [NBP1-90464]

Immunohistochemistry-Paraffin: WASHC1 Antibody [NBP1-90464]

Immunohistochemistry-Paraffin: WASHC1 Antibody [NBP1-90464] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.

Applications for WASHC1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WASHC1

Alternate Names

CXYorf1-Like Protein On Chromosome 9, FAM39E, Family With Sequence Similarity 39, Member E, FLJ00075 Protein, Protein FAM39E, WAS Protein Family Homolog 1, WASH, WASH Complex Subunit 1, WASH1

Gene Symbol

washc1

Additional WASHC1 Products

Product Documents for WASHC1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for WASHC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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