WDR12 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20888

Novus Biologicals, part of Bio-Techne
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NBP2-20888
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Chimpanzee (100%), Rhesus Macaque (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all WDR12 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human WDR12. The exact sequence is proprietary.

Localization

Nucleus; nucleolus; nucleoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for WDR12 Antibody

Immunocytochemistry/ Immunofluorescence: WDR12 Antibody [NBP2-20888]

Immunocytochemistry/ Immunofluorescence: WDR12 Antibody [NBP2-20888]

Immunocytochemistry/Immunofluorescence: WDR12 Antibody [NBP2-20888] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:200 dilution.
Western Blot: WDR12 Antibody [NBP2-20888]

Western Blot: WDR12 Antibody [NBP2-20888]

Western Blot: WDR12 Antibody [NBP2-20888] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:1000.

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with WDR12 antibody [N1C2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with WDR12 antibody [N1C2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications for WDR12 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: WDR12

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein is highly similar to the mouse WD repeat domain 12 protein at the amino acid level. The protein encoded by this gene is a component of a nucleolar protein complex that affects maturation of the large ribosomal subunit.

Alternate Names

FLJ10881, FLJ12719, FLJ12720, ribosome biogenesis protein WDR12, WD repeat domain 12, WD repeat-containing protein 12, YTM1

Gene Symbol

WDR12

UniProt

Q9GZL7 (Human)

Additional WDR12 Products

Product Documents for WDR12 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for WDR12 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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