WDR4 Antibody (5R7B6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15902

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5R7B6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all WDR4 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 250-350 of human WDR4 (P57081). AFWCQENCVALLCDGTPVVYIFQLDARRQQLVYRQQLAFQHQVWDVAFEETQGLWVLQDCQEAPLVLYRPVGDQWQSVPESTVLKKVSGVLRGNWAMLEGS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WDR4 Antibody (5R7B6)

Western Blot: WDR4 Antibody (5R7B6) [NBP3-15902]

Western Blot: WDR4 Antibody (5R7B6) [NBP3-15902]

Western Blot: WDR4 Antibody (5R7B6) [NBP3-15902] - Analysis of extracts of various cell lines, using WDR4 Rabbit mAb (NBP3-15902) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: WDR4 Antibody (5R7B6) [NBP3-15902]

Immunohistochemistry-Paraffin: WDR4 Antibody (5R7B6) [NBP3-15902]

Immunohistochemistry-Paraffin: WDR4 Antibody (5R7B6) [NBP3-15902] - Human colon carcinoma using WDR4 Rabbit mAb (NBP3-15902) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western Blot: WDR4 Antibody (5R7B6) [NBP3-15902]

Western Blot: WDR4 Antibody (5R7B6) [NBP3-15902]

Western Blot: WDR4 Antibody (5R7B6) [NBP3-15902] - Analysis of extracts of various cell lines, using WDR4 Rabbit mAb (NBP3-15902) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Applications for WDR4 Antibody (5R7B6)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: WDR4

WDR4 encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene is excluded as a candidate for a form of nonsyndromic deafness (DFNB10), but is still a candidate for other disorders mapped to 21q22.3 as well as for the development of Down syndrome phenotypes. Two transcript variants encoding the same protein have been found for this gene.

Alternate Names

tRNA (guanine-N(7)-)-methyltransferase subunit WDR4, WD repeat domain 4, 10TRM82, WD repeat-containing protein 4

Gene Symbol

WDR4

Additional WDR4 Products

Product Documents for WDR4 Antibody (5R7B6)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for WDR4 Antibody (5R7B6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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