WDR4 Antibody (OTI4G1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-74865

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4G1

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all WDR4 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human WDR4(NP_387510) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

45.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for WDR4 Antibody (OTI4G1) - Azide and BSA Free

Western Blot: WDR4 Antibody (OTI4G1)Azide and BSA Free [NBP2-74865]

Western Blot: WDR4 Antibody (OTI4G1)Azide and BSA Free [NBP2-74865]

Western Blot: WDR4 Antibody (OTI4G1) - Azide and BSA Free [NBP2-74865] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WDR4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WDR4.
Immunocytochemistry/ Immunofluorescence: WDR4 Antibody (OTI4G1) - Azide and BSA Free [NBP2-74865]

Immunocytochemistry/ Immunofluorescence: WDR4 Antibody (OTI4G1) - Azide and BSA Free [NBP2-74865]

Immunocytochemistry/Immunofluorescence: WDR4 Antibody (OTI4G1) - Azide and BSA Free [NBP2-74865] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY WDR4.
Flow Cytometry: WDR4 Antibody (OTI4G1) - Azide and BSA Free [NBP2-74865]

Flow Cytometry: WDR4 Antibody (OTI4G1) - Azide and BSA Free [NBP2-74865]

Flow Cytometry: WDR4 Antibody (OTI4G1) - Azide and BSA Free [NBP2-74865] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-WDR4 antibody, and then analyzed by flow cytometry.

Applications for WDR4 Antibody (OTI4G1) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: WDR4

WDR4 encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene is excluded as a candidate for a form of nonsyndromic deafness (DFNB10), but is still a candidate for other disorders mapped to 21q22.3 as well as for the development of Down syndrome phenotypes. Two transcript variants encoding the same protein have been found for this gene.

Alternate Names

tRNA (guanine-N(7)-)-methyltransferase subunit WDR4, WD repeat domain 4, 10TRM82, WD repeat-containing protein 4

Gene Symbol

WDR4

Additional WDR4 Products

Product Documents for WDR4 Antibody (OTI4G1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for WDR4 Antibody (OTI4G1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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