WDR4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97810

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all WDR4 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human WDR4 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for WDR4 Antibody

Western Blot: WDR4 Antibody [NBP2-97810]

Western Blot: WDR4 Antibody [NBP2-97810]

Western Blot: WDR4 Antibody [NBP2-97810] - Anti-WDR4 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 45 kDa
Immunohistochemistry-Paraffin: WDR4 Antibody [NBP2-97810]

Immunohistochemistry-Paraffin: WDR4 Antibody [NBP2-97810]

Immunohistochemistry-Paraffin: WDR4 Antibody [NBP2-97810] - Immunochemical staining of human WDR4 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Immunoprecipitation: WDR4 Antibody [NBP2-97810]

Immunoprecipitation: WDR4 Antibody [NBP2-97810]

Immunoprecipitation: WDR4 Antibody [NBP2-97810] - WDR4 was immunoprecipitated using: Lane A: 0.5 mg HeLa Whole Cell Lysate 4 ul anti-WDR4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-WDR4 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 45 kDa

Applications for WDR4 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WDR4

WDR4 encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene is excluded as a candidate for a form of nonsyndromic deafness (DFNB10), but is still a candidate for other disorders mapped to 21q22.3 as well as for the development of Down syndrome phenotypes. Two transcript variants encoding the same protein have been found for this gene.

Alternate Names

tRNA (guanine-N(7)-)-methyltransferase subunit WDR4, WD repeat domain 4, 10TRM82, WD repeat-containing protein 4

Gene Symbol

WDR4

Additional WDR4 Products

Product Documents for WDR4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for WDR4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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