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WDR5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18672

Novus Biologicals, part of Bio-Techne
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NBP3-18672-100ul
NBP3-18672-25ul

Key Product Details

Species Reactivity

Validated:

Mouse

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for WDR5 Antibody

Immunogen

Polyclonal antibody raised in rabbit against mouse Wdr5 (WD (tryptophan-aspartate) repeat domain protein 5), using two KLH-conjugated synthetic peptides containing an amino acid sequence from the central part of the protein (1).

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for WDR5 Antibody

Western Blot: WDR5 Antibody [NBP3-18672]

Western Blot: WDR5 Antibody [NBP3-18672]

Western Blot: WDR5 Antibody [NBP3-18672] - Figure 2. Western blot analysis using the antibody directed against Wdr5 (1) Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse Wdr5 , diluted 1:1,000 in BSA/PBS- Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (36 kDa) is indicated on the right.
ELISA: WDR5 Antibody [NBP3-18672]

ELISA: WDR5 Antibody [NBP3-18672]

ELISA: WDR5 Antibody [NBP3-18672] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse Wdr5 . By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:12,300.

Applications for WDR5 Antibody

Application
Recommended Usage

ELISA

1:100-1:500

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Whole Antiserum

Formulation

Whole antiserum

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: WDR5

WDR5 encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein contains 7 WD repeats. Alternatively spliced transcript variants encoding the same protein have been identified.

Long Name

WD Repeat-containing Protein 5

Alternate Names

BIG3, SWD3

Gene Symbol

WDR5

UniProt

Additional WDR5 Products

Product Documents for WDR5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for WDR5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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