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WDR53 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82758

Novus Biologicals, part of Bio-Techne
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NBP1-82758
NBP1-82758-25ul

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PVLCLNASKEGLLASGAEGGDLTAWGEDGTPLGHTRFQGADDVTSVLFSPSCPTKLYASHGETISVLDVRSLKDSLDHFHVNEEEINCLSLNQTENLLASADDSGAIKILDLENKKVIRSLKRHSNICSSV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WDR53 Antibody

Western Blot: WDR53 Antibody [NBP1-82758]

Western Blot: WDR53 Antibody [NBP1-82758]

Western Blot: WDR53 Antibody [NBP1-82758] - Analysis using Anti-WDR53 antibody NBP1-82758 (A) shows similar pattern to independent antibody NBP1-82757 (B).
Immunocytochemistry/ Immunofluorescence: WDR53 Antibody [NBP1-82758]

Immunocytochemistry/ Immunofluorescence: WDR53 Antibody [NBP1-82758]

Immunocytochemistry/Immunofluorescence: WDR53 Antibody [NBP1-82758] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
Immunohistochemistry-Paraffin: WDR53 Antibody [NBP1-82758]

Immunohistochemistry-Paraffin: WDR53 Antibody [NBP1-82758]

Immunohistochemistry-Paraffin: WDR53 Antibody [NBP1-82758] - Staining of human small intestine shows cytoplasmic positivity in glandular cells.

Applications for WDR53 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WDR53

The WDR53 gene encodes a protein containing WD domains. The function of this gene is unknown. [provided by RefSeq]

Alternate Names

G-beta repeat-containing protein, MGC12928, MGC64882, WD repeat domain 53, WD repeat-containing protein 53

Gene Symbol

WDR53

Additional WDR53 Products

Product Documents for WDR53 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for WDR53 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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