WDR57 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92586

Novus Biologicals, part of Bio-Techne
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NBP1-92586
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all WDR57 Antibodies »

Product Summary for WDR57 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GDCDNYATLKGHSGAVMELHYNTDGSMLFSASTDKTVAVWDSETGERVKRLKGHTSFVNSCYPARRGPQLVCTGSDDGTVKLWDIRKKAAIQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WDR57 Antibody

Western Blot: WDR57 Antibody [NBP1-92586]

Western Blot: WDR57 Antibody [NBP1-92586]

Western Blot: WDR57 Antibody [NBP1-92586] - Analysis in control (vector only transfected HEK293T lysate) and SNRNP40 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: WDR57 Antibody [NBP1-92586]

Immunohistochemistry-Paraffin: WDR57 Antibody [NBP1-92586]

Immunohistochemistry-Paraffin: WDR57 Antibody [NBP1-92586] - Staining of human colon shows strong cytoplasmic and nuclear positivity in glandular cells.
Western Blot: WDR57 Antibody [NBP1-92586]

Western Blot: WDR57 Antibody [NBP1-92586]

Western Blot: WDR57 Antibody [NBP1-92586] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SNRNP40 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Applications for WDR57 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WDR57

WDR57 encodes a component of the U5 small nuclear ribonucleoprotein (snRNP) particle. The U5 snRNP is part of the spliceosome, a multiprotein complex that catalyzes the removal of introns from pre-messenger RNAs. [provided by RefSeq]

Alternate Names

38 kDa-splicing factor, HPRP8BP, Prp8-binding protein, PRP8BP40K, PRPF8BPFLJ41108, RP11-490K7.3, SFP38, small nuclear ribonucleoprotein 40kDa (U5), SPF38MGC1910, U5 small nuclear ribonucleoprotein 40 kDa protein, U5 snRNP 40 kDa protein, U5 snRNP-specific 40 kDa protein, U5 snRNP-specific 40 kDa protein (hPrp8-binding), U5-40K, U5-40kD protein, WD repeat domain 57 (U5 snRNP specific), WD repeat-containing protein 57, WDR57

Gene Symbol

SNRNP40

Additional WDR57 Products

Product Documents for WDR57 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for WDR57 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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