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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 750 and 800 of human WD repeat domain 62 using the numbering given in entry NP_775907.4 (GeneID 284403).

Reactivity Notes

Mouse reactivity reported in (PMID: 22899712).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WDR62 Antibody

Immunoprecipitation: WDR62 Antibody [NB100-77302]

Immunoprecipitation: WDR62 Antibody [NB100-77302]

Immunoprecipitation: WDR62 Antibody [NB100-77302] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-WDR62 antibody NB100-77302 used for IP at 3 ug/mg lysate. WDR62 was also immunoprecipitated by rabbit anti WDR62 antibody NB100-77303 which recognizes a downstream epitope. For blotting immunoprecipitated WDR62, NB100-77303 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Applications for WDR62 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:100-1:2000
Application Notes
ICC/IFand WB were reported in scientific literature (PMID: 22899712).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: WDR62

WDR62 (WD repeat-containing 62) contains 15 WD repeats and is a member of the WD family of proteins. The WD repeat is defined by four or more repeating units of a conserved core of approximately 40 amino acids ending with tryptophan-aspartic acid (WD). WD repeats may serve as sites of protein-protein interaction for adaptor proteins and facilitate multiprotein complex formation. WD proteins are involved in a variety of cellular processes. WDR62 has been identified in a phosphoproteome analysis as a protein associated with the mitotic spindle; however its function has not been characterized.

Alternate Names

C19orf14MGC166976, chromosome 19 open reading frame 14, DKFZP434J046, DKFZp686G1024, EC 2.7.11.1, EC 2.7.7.38, FLJ33298, WD repeat domain 62, WD repeat-containing protein 62

Entrez Gene IDs

284403 (Human)

Gene Symbol

WDR62

UniProt

Additional WDR62 Products

Product Documents for WDR62 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for WDR62 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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