Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4B2

Concentration

1 mg/ml

View all WDR74 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 114-385 of human WDR74 (NP_060563) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

42.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for WDR74 Antibody (OTI4B2)

Western Blot: WDR74 Antibody (OTI4B2) [NBP2-45420]

Western Blot: WDR74 Antibody (OTI4B2) [NBP2-45420]

Western Blot: WDR74 Antibody (4B2) [NBP2-45420] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry: WDR74 Antibody (OTI4B2) [NBP2-45420]

Immunohistochemistry: WDR74 Antibody (OTI4B2) [NBP2-45420]

Immunohistochemistry: WDR74 Antibody (4B2) [NBP2-45420] - Analysis of Human breast tissue.
Western Blot: WDR74 Antibody (OTI4B2) [NBP2-45420]

Western Blot: WDR74 Antibody (OTI4B2) [NBP2-45420]

Western Blot: WDR74 Antibody (4B2) [NBP2-45420] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WDR74.
View 1 more images

Applications for WDR74 Antibody (OTI4B2)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: WDR74

WDR74, also known as WD repeat-containing protein 74, has a 385 amino acid long isoform that is 42 kDa and a short 366 amino acid isoform that is approx. 40 kDa, is located in the nucleolus and known to be required for RNA transcription, processing and/or stability during pre-implantation development and blastocyst formation in mouse. It is associated with Jervell and Lange-Nielsen syndrome, Jacobsen syndrome and Niemann-Pick disease. This protein has also shown to have an interaction with RGS2, CALR and NUDT3 in blastocyst formation, biological and RNA metabolic processes.

Alternate Names

FLJ10439, FLJ21730, NOP seven-associated protein 1, NSA1, WD repeat domain 74, WD repeat-containing protein 74

Gene Symbol

WDR74

Additional WDR74 Products

Product Documents for WDR74 Antibody (OTI4B2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for WDR74 Antibody (OTI4B2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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