WDR77 Antibody (3F10)

Novus Biologicals, part of Bio-Techne | Catalog # H00079084-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3F10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all WDR77 Antibodies »

Product Specifications

Immunogen

WDR77 (AAH01679, 1 a.a. ~ 342 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MRKETPPPLVPPAAREWNLPPNAPACMERQLEAARYRSDGALLLGASSLSGRCWAGSLWLFKDPCAAPNEGFCSAGVQTEAGVADLTWVGERGILVASDSGAVELWELDENETLIVSKFCKYEHDDIVSTVSVLSSGTQAVSGSKDICIKVWDLAQQVVLSSYRAHAAQVTCVAASPHKDSVFLSCSEDNRILLWDTRCPKPASQIGCSAPGYLPTSLAWHPQQSEVFVFGDENGTVSLVDTKSTSCVLSSAVHSQCVTGLVFSPHSVPFLASLSEDCSLAVLDSSLSELFRSQAHRDFVRDATWSPLNHSLLTTVGWDHQVVHHVVPTEPLPAPGPASVTE

Reactivity Notes

Human. Other species not tested.

Specificity

WDR77 - WD repeat domain 77

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for WDR77 Antibody (3F10)

Western Blot: WDR77 Antibody (3F10) [H00079084-M01]

Western Blot: WDR77 Antibody (3F10) [H00079084-M01]

Western Blot: WDR77 Antibody (3F10) [H00079084-M01] - WDR77 monoclonal antibody (M01), clone 3F10 Analysis of WDR77 expression in HeLa.
Immunohistochemistry-Paraffin: WDR77 Antibody (3F10) [H00079084-M01]

Immunohistochemistry-Paraffin: WDR77 Antibody (3F10) [H00079084-M01]

Immunohistochemistry-Paraffin: WDR77 Antibody (3F10) [H00079084-M01] - Analysis of monoclonal antibody to WDR77 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.
Western Blot: WDR77 Antibody (3F10) [H00079084-M01]

Western Blot: WDR77 Antibody (3F10) [H00079084-M01]

Western Blot: WDR77 Antibody (3F10) [H00079084-M01] - Analysis of WDR77 expression in transfected 293T cell line by WDR77 monoclonal antibody (M01), clone 3F10.Lane 1: WDR77 transfected lysate(36.7 KDa).Lane 2: Non-transfected lysate.
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Applications for WDR77 Antibody (3F10)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: WDR77

WDR77 is a component of the 20S PRMT5 (MIM 604045)-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins (see MIM 601061). This modification targets Sm proteins to the survival of motor neurons (SMN) complex (see MIM 600354) for assembly into small nuclear ribonucleoprotein core particles (Friesen et al., 2002 [PubMed 11756452]).[supplied by OMIM]

Alternate Names

Androgen receptor cofactor p44, MEP-50, MEP50Nbla10071, methylosome protein 50, MGC2722, p44, p44/Mep50, RP11-552M11.3, WD repeat domain 77, WD repeat-containing protein 77

Entrez Gene IDs

79084 (Human)

Gene Symbol

WDR77

UniProt

AAH01679 (Human)

Additional WDR77 Products

Product Documents for WDR77 Antibody (3F10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for WDR77 Antibody (3F10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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