WDR77 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-77305

Novus Biologicals, part of Bio-Techne
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NB100-77305
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all WDR77 Antibodies »

Product Summary for WDR77 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 292 and 342 of human WD repeat domain 77 (methylosome protein 50) using the numbering given in entry NP_077007.1 (GeneID 79084).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WDR77 Antibody

Western Blot: WDR77 Antibody [NB100-77305]

Western Blot: WDR77 Antibody [NB100-77305]

Western Blot: WDR77 Antibody [NB100-77305] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-WDR77/MEP50 antibody sed for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. WDR77/MEP50 was also immunoprecipitated by rabbit anti-WDR77/MEP50 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 1 second (B).
Immunohistochemistry: WDR77 Antibody [NB100-77305]

Immunohistochemistry: WDR77 Antibody [NB100-77305]

Immunohistochemistry: WDR77 Antibody [NB100-77305] - Sample: FFPE section of human pancreatic islet cell tumor. Antibody: Affinity purified rabbit anti- WDR77/MEP50 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB

Applications for WDR77 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5ug/mg

Western Blot

1:100-1:2000
Application Notes
Suitable for Immunofluorescence, Immunohistochemistry-Paraffin, Western blotting and Immunoprecipitation.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: WDR77

WDR77 is a component of the 20S PRMT5 (MIM 604045)-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins (see MIM 601061). This modification targets Sm proteins to the survival of motor neurons (SMN) complex (see MIM 600354) for assembly into small nuclear ribonucleoprotein core particles (Friesen et al., 2002 [PubMed 11756452]).[supplied by OMIM]

Alternate Names

Androgen receptor cofactor p44, MEP-50, MEP50Nbla10071, methylosome protein 50, MGC2722, p44, p44/Mep50, RP11-552M11.3, WD repeat domain 77, WD repeat-containing protein 77

Entrez Gene IDs

79084 (Human)

Gene Symbol

WDR77

UniProt

Q9BQA1 (Human)

Additional WDR77 Products

Product Documents for WDR77 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for WDR77 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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