West Nile Virus Envelope Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56744
Key Product Details
Species Reactivity
Virus
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1 mg/ml
Product Specifications
Immunogen
A synthetic peptide corresponding to 73-86 (CPTMGEAHNEKRAD) of West Nile Virus Envelope glycoprotein (NC_001563).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for West Nile Virus Envelope Antibody
Western Blot: West Nile Virus Envelope Antibody [NB100-56744]
Western Blot: West Nile Virus Envelope Antibody [NB100-56744] - Analysis using the Azide Free version of NB100-56744. Detection of West Nile Virus Envelope in (A) untransfected and (B) transfected cells using this antibody.Immunohistochemistry-Paraffin: West Nile Virus Envelope Antibody [NB100-56744]
Immunohistochemistry-Paraffin: West Nile Virus Envelope Antibody [NB100-56744] - Analysis using the Azide Free version of NB100-56744. Staining of West Nile Virus Envelope in bird liver using this antibody at 1 ug/ml, incubated for 30 minutes. A Ventana brand second step antibody conjugated to biotin was applied followed with streptavidin-AKP. The substrate was Fast Red chromagen. The antigen retrieval method used to obtain staining: Ventana Protease 2 for three minutes at room temperature with a Parafilm coverslip. (using an autostainer which keeps residual fluid on the slides during every step, so the final concentration is approximately 2.5 times more dilute). Image and protocol used to generate this image are courtesy of Angela Ellis, University of Georgia College of Veterinary Medicine.Applications for West Nile Virus Envelope Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
reported in scientific literature (PMID 24920798)
Immunohistochemistry
1 ug/ml
Immunohistochemistry-Paraffin
1 ug/ml
Western Blot
1-3 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: West Nile Virus Envelope
UniProt
Additional West Nile Virus Envelope Products
Product Documents for West Nile Virus Envelope Antibody
Product Specific Notices for West Nile Virus Envelope Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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