West Nile Virus MP Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56743
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Virus
Cited:
Human, Mouse, Hamster, Primate - Macaca mulatta (Rhesus Macaque), Virus
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A synthetic peptide corresponding to amino acid residues 8-27 (GESTLANKKGAWLDSTKATR) of West Nile Virus MP. Genbank accession no. NP_776013.
Reactivity Notes
West Nile Virus glycoprotein M (Genbank accession no. NP_776013).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for West Nile Virus MP Antibody - BSA Free
Western Blot: West Nile Virus MP AntibodyBSA Free [NB100-56743]
Western Blot: West Nile Virus MP Antibody - BSA Free [NB100-56743] - Analysis of West Nile Virus West Nile Virus MP in (A) untransfected and (B) transfected mouse melanoma cells using NB100-56743 at 1 ug/ml.Immunohistochemistry-Paraffin: West Nile Virus MP Antibody - BSA Free [NB100-56743]
Immunohistochemistry-Paraffin: West Nile Virus MP Antibody - BSA Free [NB100-56743] - Analysis of West Nile Virus MP in bird liver using this antibody at 1 ug/ml, incubated for 30 minutes. A Ventana brand second step antibody conjugated to biotin was applied followed with streptavidin-AKP. The substrate was Fast Red chromagen. The antigen retrieval method used to obtain staining: Ventana Protease 2 for three minutes at room temperature with a Parafilm coverslip. (using an autostainer which keeps residual fluid on the slides during every step, so the final concentration is approximately 2.5 times more dilute). Image and protocol used to generate this image are courtesy of Angela Ellis, University of Georgia College of Veterinary Medicine.Applications for West Nile Virus MP Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:20-1:1000. Use reported in scientific literature (Tan et al (2009))
Immunohistochemistry
1:20-1:1000
Immunohistochemistry-Paraffin
1.0 ug/ ml
Immunoprecipitation
reported in scientific literature (Tan et al (2009))
Western Blot
0.5-2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: West Nile Virus MP
Additional West Nile Virus MP Products
Product Documents for West Nile Virus MP Antibody - BSA Free
Product Specific Notices for West Nile Virus MP Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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