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WHSC2 Antibody (6B11H8) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37394

Novus Biologicals, part of Bio-Techne
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NBP2-37394-0.025ml
NBP2-37394

Key Product Details

Species Reactivity

Human, Rat

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 6B11H8

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Purified recombinant fragment of human WHSC2 (AA: 280-511) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

57.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for WHSC2 Antibody (6B11H8) - BSA Free

Western Blot: WHSC2 Antibody (6B11H8)BSA Free [NBP2-37394]

Western Blot: WHSC2 Antibody (6B11H8)BSA Free [NBP2-37394]

Western Blot: WHSC2 Antibody (6B11H8) [NBP2-37394] - Western blot analysis using WHSC2 mouse mAb against Jurkat (1), HeLa (2), HEK293 (3), A549 (5), SPC-A-1 (6) cell lysate, and Rat brain (4) tissue lysate.
Immunocytochemistry/ Immunofluorescence: WHSC2 Antibody (6B11H8) - BSA Free [NBP2-37394]

Immunocytochemistry/ Immunofluorescence: WHSC2 Antibody (6B11H8) - BSA Free [NBP2-37394]

Immunocytochemistry/Immunofluorescence: WHSC2 Antibody (6B11H8) [NBP2-37394] - Immunofluorescence analysis of HeLa cells using WHSC2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry: WHSC2 Antibody (6B11H8) - BSA Free [NBP2-37394]

Immunohistochemistry: WHSC2 Antibody (6B11H8) - BSA Free [NBP2-37394]

Immunohistochemistry: WHSC2 Antibody (6B11H8) [NBP2-37394] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using WHSC2 mouse mAb with DAB staining.

Applications for WHSC2 Antibody (6B11H8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WHSC2

WHSC2 is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation. [provided by RefSeq]

Alternate Names

FLJ10442, negative elongation factor A, NELFA, NELF-AFLJ25112, Wolf-Hirschhorn syndrome candidate 2, Wolf-Hirschhorn syndrome candidate 2 protein

Gene Symbol

WHSC2

Additional WHSC2 Products

Product Documents for WHSC2 Antibody (6B11H8) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for WHSC2 Antibody (6B11H8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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