Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all WHSC2 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 499 and 549 of human negative elongation factor A using the numbering given in entry BAB18651.1 (GeneID 7469).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WHSC2 Antibody

Western Blot: WHSC2 Antibody [NBP1-05975]

Western Blot: WHSC2 Antibody [NBP1-05975]

Western Blot: WHSC2 Antibody [NBP1-05975] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-NELFA antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.
Immunohistochemistry-Paraffin: WHSC2 Antibody [NBP1-05975]

Immunohistochemistry-Paraffin: WHSC2 Antibody [NBP1-05975]

Immunohistochemistry-Paraffin: WHSC2 Antibody [NBP1-05975] - Sample: FFPE section of human placenta. Antibody: Affinity purified rabbit anti-NELFA used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Applications for WHSC2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500-1:2000

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: WHSC2

This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation. [provided by RefSeq]

Alternate Names

FLJ10442, negative elongation factor A, NELFA, NELF-AFLJ25112, Wolf-Hirschhorn syndrome candidate 2, Wolf-Hirschhorn syndrome candidate 2 protein

Entrez Gene IDs

7469 (Human)

Gene Symbol

WHSC2

UniProt

BAB18651.1 (Human)

Additional WHSC2 Products

Product Documents for WHSC2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for WHSC2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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