Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5E2

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all WIBG Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human WIBG (NP_115721) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for WIBG Antibody (OTI5E2) - Azide and BSA Free

Western Blot: WIBG Antibody (OTI5E2)Azide and BSA Free [NBP2-74868]

Western Blot: WIBG Antibody (OTI5E2)Azide and BSA Free [NBP2-74868]

Western Blot: WIBG Antibody (OTI5E2) - Azide and BSA Free [NBP2-74868] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WIBG.
Immunohistochemistry: WIBG Antibody (OTI5E2) - Azide and BSA Free [NBP2-74868]

Immunohistochemistry: WIBG Antibody (OTI5E2) - Azide and BSA Free [NBP2-74868]

Immunohistochemistry: WIBG Antibody (OTI5E2) - Azide and BSA Free [NBP2-74868] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: WIBG Antibody (OTI5E2) - Azide and BSA Free [NBP2-74868]

Immunohistochemistry: WIBG Antibody (OTI5E2) - Azide and BSA Free [NBP2-74868]

Immunohistochemistry: WIBG Antibody (OTI5E2) - Azide and BSA Free [NBP2-74868] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
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Applications for WIBG Antibody (OTI5E2) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: WIBG

Key regulator of the exon junction complex (EJC), a multiprotein complex that associates immediately upstreamof the exon-exon junction on mRNAs and serves as a positional landmarks for the intron exon structure of genes anddirects post-transcriptional processes in the cytoplasm such as mRNA export, nonsense-mediated mRNA decay (NMD) ortranslation. Acts as a EJC disassembly factor, allowing translation-dependent EJC removal and recycling by disruptingmature EJC from spliced mRNAs. Its association with the 40S ribosomal subunit probably prevents atranslation-independent disassembly of the EJC from spliced mRNAs, by restricting its activity to mRNAs that have beentranslated. Interferes with NMD and enhances translation of spliced mRNAs, probably by antagonizing EJC functions. Maybind RNA; the relevance of RNA-binding remains unclear in vivo, RNA-binding was detected by PubMed:14968132, whilePubMed:19410547 did not detect RNA-binding activity independently of the EJC

Alternate Names

MGC13064, partner of Y14 and mago, PYMProtein wibg homolog, within bgcn homolog (Drosophila)

Gene Symbol

PYM1

Additional WIBG Products

Product Documents for WIBG Antibody (OTI5E2) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for WIBG Antibody (OTI5E2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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