WIBG Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92596

Novus Biologicals, part of Bio-Techne
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NBP1-92596
NBP1-92596-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all WIBG Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KEKRRQQQEKGEAEALSRTLDKVSLEETAQLPSAPQGSRAAPTAASDQPDSAATTEKAKKIKNLK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WIBG Antibody

Western Blot: WIBG Antibody [NBP1-92596]

Western Blot: WIBG Antibody [NBP1-92596]

Western Blot: WIBG Antibody [NBP1-92596] - Analysis in control (vector only transfected HEK293T lysate) and WIBG over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: WIBG Antibody [NBP1-92596]

Immunohistochemistry-Paraffin: WIBG Antibody [NBP1-92596]

Immunohistochemistry-Paraffin: WIBG Antibody [NBP1-92596] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.

Applications for WIBG Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WIBG

Key regulator of the exon junction complex (EJC), a multiprotein complex that associates immediately upstreamof the exon-exon junction on mRNAs and serves as a positional landmarks for the intron exon structure of genes anddirects post-transcriptional processes in the cytoplasm such as mRNA export, nonsense-mediated mRNA decay (NMD) ortranslation. Acts as a EJC disassembly factor, allowing translation-dependent EJC removal and recycling by disruptingmature EJC from spliced mRNAs. Its association with the 40S ribosomal subunit probably prevents atranslation-independent disassembly of the EJC from spliced mRNAs, by restricting its activity to mRNAs that have beentranslated. Interferes with NMD and enhances translation of spliced mRNAs, probably by antagonizing EJC functions. Maybind RNA; the relevance of RNA-binding remains unclear in vivo, RNA-binding was detected by PubMed:14968132, whilePubMed:19410547 did not detect RNA-binding activity independently of the EJC

Alternate Names

MGC13064, partner of Y14 and mago, PYMProtein wibg homolog, within bgcn homolog (Drosophila)

Gene Symbol

PYM1

Additional WIBG Products

Product Documents for WIBG Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for WIBG Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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