WIF-1 Antibody (JE41-39)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33102
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE41-39
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human WIF-1 aa 330-379 / 379. (Uniprot: Q9Y5W5)
Localization
Secreted.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for WIF-1 Antibody (JE41-39)
Western Blot: WIF-1 Antibody (JE41-39) [NBP3-33102]
Western Blot: WIF-1 Antibody (JE41-39) [NBP3-33102] - Western blot analysis of WIF-1 on different lysates with Rabbit anti-WIF-1 antibody (NBP3-33102) at 1/1,000 dilution.Lane 1: HT-29 cell lysate (20 ug/Lane)
Lane 2: HeLa cell lysate (20 ug/Lane)
Lane 3: SW480 cell lysate (20 ug/Lane)
Lane 4: Caco-2 cell lysate (20 ug/Lane)
Lane 5: NIH/3T3 cell lysate (20 ug/Lane)
Lane 6: Mouse heart tissue lysate (40 ug/Lane)
Lane 7: Mouse lung tissue lysate (40 ug/Lane)
Lane 8: Rat lung tissue lysate (40 ug/Lane)
Lane 9: Human lung tissue lysate (40 ug/Lane)
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33102) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.
Applications for WIF-1 Antibody (JE41-39)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: WIF-1
Long Name
Wnt Inhibitory Factor
Alternate Names
WIF1
Gene Symbol
WIF1
Additional WIF-1 Products
Product Documents for WIF-1 Antibody (JE41-39)
Product Specific Notices for WIF-1 Antibody (JE41-39)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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