WISP2 Antibody (3D10)

Novus Biologicals, part of Bio-Techne | Catalog # H00008839-M09

Novus Biologicals, part of Bio-Techne
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H00008839-M09
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3D10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all WISP2/CCN5 Antibodies »

Product Specifications

Immunogen

WISP2 (AAH17782.1, 24 a.a. ~ 250 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QLCPTPCTCPWPPPRCPLGVPLVLDGCGCCRVCARRLGEPCDQLHVCDASQGLVCQPGAGPGGRGALCLLAEDDCSCEVNGRLYREGETFQPHCSIRCRCEDGGFTCVPLCSEDVRLPSWDCPHPRRVEVLGKCCPEWVCGQGGGLGTQPLPAQGPQFSGLVSSLPPGVPCPEWSTAWGPCSTTCGLGMATRVSNQNRFCRLETQRRLCLSRPCPPSRGRSPQNSAF

Specificity

WISP2 - WNT1 inducible signaling pathway protein 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for WISP2 Antibody (3D10)

Western Blot: WISP2 Antibody (3D10) [H00008839-M09]

Western Blot: WISP2 Antibody (3D10) [H00008839-M09]

Western Blot: WISP2 Antibody (3D10) [H00008839-M09] - Analysis of WISP2 expression in transfected 293T cell line by WISP2 monoclonal antibody (M09), clone 3D10. Lane 1: WISP2 transfected lysatE (26.8 KDa). Lane 2: Non-transfected lysate.
Immunoprecipitation: WISP2 Antibody (3D10) [H00008839-M09]

Immunoprecipitation: WISP2 Antibody (3D10) [H00008839-M09]

Immunoprecipitation: WISP2 Antibody (3D10) [H00008839-M09] - Analysis of WISP2 transfected lysate using anti-WISP2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with WISP2 rabbit polyclonal antibody.

Applications for WISP2 Antibody (3D10)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: WISP2/CCN5

This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like (CT) domain. The encoded protein lacks the CT domain which is implicated in dimerization and heparin binding. It is 72% identical to the mouse protein at the amino acid level. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Its expression in colon tumors is reduced while the other two WISP members are overexpressed in colon tumors. It is expressed at high levels in bone tissue, and may play an important role in modulating bone turnover.

Long Name

WNT1 Inducible Signaling Pathway Protein 2

Alternate Names

CCN5, CTGF-L, CTGFL

Entrez Gene IDs

8839 (Human)

Gene Symbol

CCN5

OMIM

603399 (Human)

UniProt

AAH17782 (Human)

Additional WISP2/CCN5 Products

Product Documents for WISP2 Antibody (3D10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for WISP2 Antibody (3D10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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