WIZ Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-21059
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Predicted:
Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Flow Cytometry, Peptide ELISA, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-RSYIQGGRPFTKKFR corresponding to internal region according to NP_067064.2.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 23213215).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for WIZ Antibody
![Western Blot: WIZ Antibody [NBP1-21059]](https://resources.bio-techne.com/images/products/WIZ-Antibody-Western-Blot-NBP1-21059-img0001.jpg "Western Blot: WIZ Antibody [NBP1-21059]")
Western Blot: WIZ Antibody [NBP1-21059]
Western Blot: WIZ Antibody [NBP1-21059] - Human Cerebellum lysate (A) + Blocking peptide (B) (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence![Flow Cytometry: WIZ Antibody [NBP1-21059]](https://resources.bio-techne.com/images/products/WIZ-Antibody-Flow-Cytometry-NBP1-21059-img0002.jpg "Flow Cytometry: WIZ Antibody [NBP1-21059]")
Flow Cytometry: WIZ Antibody [NBP1-21059]
Flow Cytometry: WIZ Antibody [NBP1-21059] - Paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Antibody at 10 ug/mL. Primary incubation 1hr followed by Alexa Fluor 488 secondary antibody at 1 ug/mL. IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.Applications for WIZ Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/mL
Peptide ELISA
Detection limit 1:2000
Western Blot
0.3 - 1 ug/mL
Application Notes
Western blot: Approx. 75 kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 85.4 kDa according to NP_067064.2). An additional band of unknown identity was also consistently observed at 38 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: WIZ
Alternate Names
widely interspaced zinc finger motifs, widely-interspaced zinc finger motifs, Widely-interspaced zinc finger-containing protein, WIZ zinc finger, Zinc finger protein 803, ZNF803protein Wiz
Gene Symbol
WIZ
UniProt
Additional WIZ Products
Product Documents for WIZ Antibody
Product Specific Notices for WIZ Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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