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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # M42-P3B10

Format

BSA Free

Concentration

1 mg/ml

Product Summary for WNK1 Antibody (M42-P3B10) - BSA Free

Immunogen

Ovalbumin-conjugated synthetic peptide KSISNPPGSN-peptide immunogen is identical in mouse (Mus musculus), rat (Rattus norvegicus), sheep (Ovis aries), bovine (Bos taurus), canine (Canis familiaris), porcine (Sus scrofa), equine (Equus caballus) and chichen (Gallus gallus).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

250 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for WNK1 Antibody (M42-P3B10) - BSA Free

Western Blot: WNK1 Antibody (M42-P3B10)BSA Free [NBP2-50364]

Western Blot: WNK1 Antibody (M42-P3B10)BSA Free [NBP2-50364]

Western Blot: WNK1 Antibody (M42-P3B10) [NBP2-50364] - Detection of WNK1 in a HeLa cell lysate. A 250kDa protein was detected.

Applications for WNK1 Antibody (M42-P3B10) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunohistochemistry

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Positive control(s): HeLa cell lysate.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WNK1

WNK lysine deficient protein kinase 1 (WNK1) is a cytoplasmic serine-threonine kinase expressed in the distal nephron which likely plays an important role in hypertension. WNK1 is responsible for controling Na+ and Cl- ion transport by inhibiting the activity of other related protein kinases, such as WNK4.

WNK1 mutations have been associated with Gordon hyperkalemia-hypertension syndrome (pseudohypoaldosteronism type II) and congenital sensory neuropathy.

WNK1 is also known as Human Accelerated Region 5, and is thought to have played a key role in differentiating humans from apes.

Long Name

WNK Lysine-deficient Protein Kinase 1

Alternate Names

KDP, PHA2C, PRKWNK1

Gene Symbol

WNK1

Additional WNK1 Products

Product Documents for WNK1 Antibody (M42-P3B10) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for WNK1 Antibody (M42-P3B10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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