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WNK2 Antibody (2E10)

Novus Biologicals, part of Bio-Techne | Catalog # H00065268-M01

Novus Biologicals, part of Bio-Techne
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H00065268-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2E10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

WNK2 (NP_006639, 2118 a.a. ~ 2217 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KGTFTDDLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARAMTAPRAGVGMPRLPPAPGPLSTTVIPGAAPTLSVPTPDPESEKPD

Reactivity Notes

Human. Other species not tested.

Specificity

WNK2 - WNK lysine deficient protein kinase 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for WNK2 Antibody (2E10)

Immunohistochemistry-Paraffin: WNK2 Antibody (2E10) [H00065268-M01]

Immunohistochemistry-Paraffin: WNK2 Antibody (2E10) [H00065268-M01]

Immunohistochemistry-Paraffin: WNK2 Antibody (2E10) [H00065268-M01] - Analysis of monoclonal antibody to WNK2 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.
Sandwich ELISA: WNK2 Antibody (2E10) [H00065268-M01] - Detection limit for recombinant GST tagged WNK2 is approximately 0.1ng/ml as a capture antibody.

Applications for WNK2 Antibody (2E10)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: WNK2

The protein encoded by this gene is a cytoplasmic serine-threonine kinase that contains cysteine in place of the lysine found at the conserved ATP-binding location in subdomain II of protein kinases. Since this protein does have kinase activity, it is possible that another lysine in the kinase subdomain I can substitute for the missing conserved lysine.

Alternate Names

EC 2.7.11, EC 2.7.11.1, PRK, PRKWNK2, Protein kinase with no lysine 2, SDCCAG43, serine/threonine-protein kinase WNK2, Serologically defined colon cancer antigen 43lysine deficient 2, WNK lysine deficient protein kinase 2

Entrez Gene IDs

65268 (Human)

Gene Symbol

WNK2

OMIM

606249 (Human)

Additional WNK2 Products

Product Documents for WNK2 Antibody (2E10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for WNK2 Antibody (2E10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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