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Wnt-10a Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33039

Novus Biologicals, part of Bio-Techne
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NBP1-33039

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (96%), Mouse (96%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Wnt-10a. The exact sequence is proprietary.

Reactivity Notes

Chicken (83%).

Localization

Secreted, extracellular space, extracellular matrix

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Wnt-10a Antibody

Immunocytochemistry/ Immunofluorescence: Wnt-10a Antibody [NBP1-33039]

Immunocytochemistry/ Immunofluorescence: Wnt-10a Antibody [NBP1-33039]

Immunocytochemistry/Immunofluorescence: Wnt-10a Antibody [NBP1-33039] - SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: WNT10A protein stained by WNT10A antibody [N1C1-2] diluted at 1:500. Blue: Hoechst 33342 staining.
Wnt-10a Antibody

Western Blot: Wnt-10a Antibody [NBP1-33039] -

Western Blot: Wnt-10a Antibody [NBP1-33039] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt-10a antibody [N1C1-2] (NBP1-33039) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications for Wnt-10a Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Wnt-10a

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is strongly expressed in the cell lines of promyelocytic leukemia and Burkitt's lymphoma. In addition, it and another family member, the WNT6 gene, are strongly coexpressed in colorectal cancer cell lines. The gene over expression may play key roles in carcinogenesis through activation of the WNT-beta-catenin-TCF signaling pathway. This gene and the WNT6 gene are clustered in the chromosome 2q35 region.

Long Name

Wingless-type MMTV Integration Site Family Member 10A

Alternate Names

Wnt10a

Gene Symbol

WNT10A

Additional Wnt-10a Products

Product Documents for Wnt-10a Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Wnt-10a Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.