Wnt-4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1006

Novus Biologicals, part of Bio-Techne
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NB100-1006
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Wnt-4 Antibodies »

Product Summary for Wnt-4 Antibody

Immunogen

Peptide with sequence C-SNWLYLAKLSSVGS corresponding to internal region according to NP_110388.2.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat.

Localization

Possibly secreted and associates with the extracellular matrix.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Wnt-4 Antibody

Western Blot: Wnt-4 Antibody [NB100-1006]

Western Blot: Wnt-4 Antibody [NB100-1006]

Western Blot: Wnt-4 Antibody [NB100-1006] - (1ug/ml) staining of Jurkat (A) and negative control U937 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Wnt-4 Antibody [NB100-1006]

Immunocytochemistry/ Immunofluorescence: Wnt-4 Antibody [NB100-1006]

Immunocytochemistry/Immunofluorescence: Wnt-4 Antibody [NB100-1006] - analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue)

Applications for Wnt-4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Peptide ELISA

Detection limit 1:4000

Western Blot

1:100-1:2000
Application Notes
WB: Approx. 40+35 kDa band observed in Jurkat lysates (calculated MW of 39.1 kDa band according to NP_110388.2 Negative Control: U937 cell lysate.).
Immunofluorescence: Strong expression of the protein seen in the Vesicle of HeLa and MCF7 cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Wnt-4

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT4 is a member of the WNT gene family, and is the first signaling molecule shown to influence the sex-determination cascade. It encodes a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. This gene and DAX1, a gene known to antagonize the testis-determining factor, play a concerted role in both the control of female development and the prevention of testes formation. WNT4 and another two family members, WNT2 and WNT7B, may be associated with abnormal proliferation in breast tissue.

Long Name

Wingless-type MMTV Integration Site Family, Member 4

Alternate Names

Wnt4

Entrez Gene IDs

54361 (Human); 22417 (Mouse)

Gene Symbol

WNT4

UniProt

NP_110388. (Human)

Additional Wnt-4 Products

Product Documents for Wnt-4 Antibody

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Certificate of Analysis

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Product Specific Notices for Wnt-4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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