Wnt-4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20909

Novus Biologicals, part of Bio-Techne
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NBP2-20909
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Xenopus (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Wnt-4 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Wnt-4. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Japanese medaka (85%), Chicken (89%), Xenopus laevis (90%).

Localization

Secreted; extracellular space; extracellular matrix

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Wnt-4 Antibody

Western Blot: Wnt-4 Antibody [NBP2-20909]

Western Blot: Wnt-4 Antibody [NBP2-20909]

Western Blot: Wnt-4 Antibody [NBP2-20909] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with WNT4 antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Wnt-4 Antibody [NBP2-20909]

Immunohistochemistry-Paraffin: Wnt-4 Antibody [NBP2-20909]

Immunohistochemistry-Paraffin: Wnt-4 Antibody [NBP2-20909] - Mouse kidney. WNT4 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: Wnt-4 Antibody [NBP2-20909]

Western Blot: Wnt-4 Antibody [NBP2-20909]

Western Blot: Wnt-4 Antibody [NBP2-20909] - Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysates B: WNT4 transfected 293T lysates 10% SDS PAGE diluted at 1:5000
View 3 more images

Applications for Wnt-4 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Wnt-4

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and is the first signaling molecule shown to influence the sex-determination cascade. It encodes a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. This gene and a nuclear receptor known to antagonize the testis-determining factor play a concerted role in both the control of female development and the prevention of testes formation. This gene and another two family members, WNT2 and WNT7B, may be associated with abnormal proliferation in breast tissue. Mutations in this gene can result in Rokitansky-Kuster-Hauser syndrome and in SERKAL syndrome. [provided by RefSeq]

Long Name

Wingless-type MMTV Integration Site Family, Member 4

Alternate Names

Wnt4

Gene Symbol

WNT4

UniProt

P56705 (Human)

Additional Wnt-4 Products

Product Documents for Wnt-4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Wnt-4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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