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Wnt-6 Antibody (HL1568) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25754

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25754

Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (93%), Mouse (93%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1568

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Wnt-6. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Wnt-6 Antibody (HL1568) - Azide and BSA Free

Wnt-6 Antibody (HL1568) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Wnt-6 Antibody (HL1568) - Azide and BSA Free [NBP3-25754] -

Immunocytochemistry/Immunofluorescence: Wnt-6 Antibody (HL1568) - Azide and BSA Free [NBP3-25754] - Wnt6 antibody [HL1568] detects Wnt6 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Wnt6 stained by Wnt6 antibody [HL1568] (NBP3-25754) diluted at 1:500. Blue: Fluoroshield with DAPI .
Wnt-6 Antibody (HL1568) - Azide and BSA Free

Immunohistochemistry-Paraffin: Wnt-6 Antibody (HL1568) - Azide and BSA Free [NBP3-25754] -

Immunohistochemistry-Paraffin: Wnt-6 Antibody (HL1568) - Azide and BSA Free [NBP3-25754] - Wnt6 antibody [HL1568] detects Wnt6 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat adrenal gland. Wnt6 stained by Wnt6 antibody [HL1568] (NBP3-25754) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Wnt-6 Antibody (HL1568) - Azide and BSA Free

Western Blot: Wnt-6 Antibody (HL1568) - Azide and BSA Free [NBP3-25754] -

Western Blot: Wnt-6 Antibody (HL1568) - Azide and BSA Free [NBP3-25754] - Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt6 antibody [HL1568] (NBP3-25754) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Wnt-6 Antibody (HL1568) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Wnt-6

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is overexpressed in cervical cancer cell line and strongly coexpressed with another family member, WNT10A, in colorectal cancer cell line. The gene overexpression may play key roles in carcinogenesis. This gene and the WNT10A gene are clustered in the chromosome 2q35 region. The protein encoded by this gene is 97% identical to the mouse Wnt6 protein at the amino acid level. [provided by RefSeq]

Long Name

Wingless-type MMTV Integration Site Family, Member 6

Alternate Names

Wnt6

Gene Symbol

WNT6

Additional Wnt-6 Products

Product Documents for Wnt-6 Antibody (HL1568) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Wnt-6 Antibody (HL1568) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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